Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2240948..2241474 Replicon chromosome
Accession NZ_CP018801
Organism Escherichia coli strain tolC-

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag BTD92_RS11810 Protein ID WP_000323025.1
Coordinates 2241187..2241474 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag BTD92_RS11805 Protein ID WP_000534858.1
Coordinates 2240948..2241187 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTD92_RS11735 2235975..2236742 - 768 Protein_2105 exonuclease -
BTD92_RS11740 2236835..2237026 - 192 WP_001083297.1 lysis protein YdfD -
BTD92_RS11745 2237023..2237211 - 189 WP_000854559.1 cell division inhibition protein DicB -
BTD92_RS11755 2237779..2237997 - 219 WP_001171942.1 hypothetical protein -
BTD92_RS11765 2238157..2238312 - 156 WP_000379575.1 DUF1391 family protein -
BTD92_RS11770 2238479..2238886 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
BTD92_RS11775 2238970..2239200 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
BTD92_RS11780 2239184..2239462 + 279 Protein_2112 hypothetical protein -
BTD92_RS11785 2239497..2239646 + 150 WP_011443592.1 hypothetical protein -
BTD92_RS11790 2240083..2240415 - 333 WP_001301033.1 protein FlxA -
BTD92_RS11800 2240618..2240923 - 306 WP_001326990.1 hypothetical protein -
BTD92_RS11805 2240948..2241187 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
BTD92_RS11810 2241187..2241474 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
BTD92_RS11815 2241546..2241701 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
BTD92_RS11820 2241918..2242169 + 252 WP_000980994.1 hypothetical protein -
BTD92_RS11825 2242236..2242514 + 279 WP_012304870.1 hypothetical protein -
BTD92_RS11830 2242516..2243565 + 1050 WP_001265198.1 DUF968 domain-containing protein -
BTD92_RS11835 2243579..2244331 + 753 WP_001047135.1 antitermination protein -
BTD92_RS26185 2244609..2244698 - 90 WP_120795389.1 hypothetical protein -
BTD92_RS11840 2244753..2244965 - 213 WP_000087756.1 cold shock-like protein CspF -
BTD92_RS11845 2245266..2245481 + 216 WP_000066484.1 cold shock-like protein CspB -
BTD92_RS26740 2245845..2246015 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
BTD92_RS11850 2246235..2246450 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2221021..2255818 34797


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T70553 WP_000323025.1 NZ_CP018801:2241187-2241474 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T70553 NZ_CP018801:2241187-2241474 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT70553 WP_000534858.1 NZ_CP018801:2240948-2241187 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT70553 NZ_CP018801:2240948-2241187 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References