224831

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1640150..1640676 Replicon chromosome
Accession NZ_CP087136
Organism Escherichia coli strain JW5503

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LOX89_RS08120 Protein ID WP_000323025.1
Coordinates 1640150..1640437 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LOX89_RS08125 Protein ID WP_000534858.1
Coordinates 1640437..1640676 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX89_RS08070 (1635174) 1635174..1635389 - 216 WP_000839590.1 phage lysis protein EssD -
LOX89_RS08075 (1635609) 1635609..1635779 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
LOX89_RS08080 (1636143) 1636143..1636358 - 216 WP_000066484.1 cold shock-like protein CspB -
LOX89_RS08085 (1636659) 1636659..1636871 + 213 WP_000087756.1 cold shock-like protein CspF -
LOX89_RS08090 (1636926) 1636926..1637015 + 90 WP_120795389.1 hypothetical protein -
LOX89_RS08095 (1637293) 1637293..1638045 - 753 WP_001047135.1 antitermination protein -
LOX89_RS08100 (1638059) 1638059..1639108 - 1050 WP_001393597.1 DUF968 domain-containing protein -
LOX89_RS08105 (1639110) 1639110..1639388 - 279 WP_012304870.1 hypothetical protein -
LOX89_RS08110 (1639455) 1639455..1639706 - 252 WP_000980994.1 protein Rem -
LOX89_RS08115 (1639923) 1639923..1640078 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
LOX89_RS08120 (1640150) 1640150..1640437 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LOX89_RS08125 (1640437) 1640437..1640676 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LOX89_RS08130 (1640701) 1640701..1641006 + 306 WP_001326990.1 protein YdfV -
LOX89_RS08135 (1641209) 1641209..1641541 + 333 WP_001301033.1 FlxA-like family protein -
LOX89_RS08140 (1641978) 1641978..1642127 - 150 WP_011443592.1 protein YdfW -
LOX89_RS08145 (1642162) 1642162..1642440 - 279 Protein_1597 protein YdfX -
LOX89_RS08150 (1642424) 1642424..1642654 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
LOX89_RS08155 (1642738) 1642738..1643145 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
LOX89_RS08160 (1643312) 1643312..1643467 + 156 WP_000379575.1 DUF1391 family protein -
LOX89_RS22565 (1643469) 1643469..1643597 + 129 WP_000344964.1 protein YdfB -
LOX89_RS08165 (1643627) 1643627..1643845 + 219 WP_001171942.1 protein YdfC -
LOX89_RS08170 (1644413) 1644413..1644601 + 189 WP_000854559.1 cell division inhibition protein DicB -
LOX89_RS08175 (1644598) 1644598..1644789 + 192 WP_001083297.1 lysis protein YdfD -
LOX89_RS08180 (1644882) 1644882..1645649 + 768 Protein_1605 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1625806..1652674 26868


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T224831 WP_000323025.1 NZ_CP087136:c1640437-1640150 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T224831 NZ_CP120563:963287-963694 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTAATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTACGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT224831 WP_000534858.1 NZ_CP087136:c1640676-1640437 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT224831 NZ_CP120563:963040-963306 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References