246447

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2222126..2222652 Replicon chromosome
Accession NZ_CP097882
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag NAG70_RS10735 Protein ID WP_000323025.1
Coordinates 2222365..2222652 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag NAG70_RS10730 Protein ID WP_000534858.1
Coordinates 2222126..2222365 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG70_RS10670 (2217153) 2217153..2217920 - 768 Protein_2082 exonuclease -
NAG70_RS10675 (2218013) 2218013..2218204 - 192 WP_001083297.1 lysis protein YdfD -
NAG70_RS10680 (2218201) 2218201..2218389 - 189 WP_000854559.1 cell division inhibition protein DicB -
NAG70_RS10685 (2218957) 2218957..2219175 - 219 WP_001171942.1 protein YdfC -
NAG70_RS10690 (2219205) 2219205..2219333 - 129 WP_000344964.1 protein YdfB -
NAG70_RS10695 (2219335) 2219335..2219490 - 156 WP_000379575.1 DUF1391 family protein -
NAG70_RS10700 (2219657) 2219657..2220064 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
NAG70_RS10705 (2220148) 2220148..2220378 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
NAG70_RS10710 (2220362) 2220362..2220640 + 279 Protein_2090 hypothetical protein -
NAG70_RS10715 (2220675) 2220675..2220824 + 150 WP_011443592.1 protein YdfW -
NAG70_RS10720 (2221261) 2221261..2221593 - 333 WP_001301033.1 FlxA-like family protein -
NAG70_RS10725 (2221796) 2221796..2222101 - 306 WP_001326990.1 protein YdfV -
NAG70_RS10730 (2222126) 2222126..2222365 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
NAG70_RS10735 (2222365) 2222365..2222652 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
NAG70_RS10740 (2222724) 2222724..2222879 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
NAG70_RS10745 (2223096) 2223096..2223347 + 252 WP_000980994.1 protein Rem -
NAG70_RS10750 (2223414) 2223414..2223692 + 279 WP_012304870.1 hypothetical protein -
NAG70_RS10755 (2223694) 2223694..2224743 + 1050 WP_001393597.1 DUF968 domain-containing protein -
NAG70_RS10760 (2224757) 2224757..2225509 + 753 WP_001047135.1 antitermination protein -
NAG70_RS10765 (2225787) 2225787..2225876 - 90 WP_120795389.1 hypothetical protein -
NAG70_RS10770 (2225931) 2225931..2226143 - 213 WP_000087756.1 cold shock-like protein CspF -
NAG70_RS10775 (2226444) 2226444..2226659 + 216 WP_000066484.1 cold shock-like protein CspB -
NAG70_RS10780 (2227023) 2227023..2227193 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
NAG70_RS10785 (2227413) 2227413..2227628 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2211254..2239646 28392


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T246447 WP_000323025.1 NZ_CP097882:2222365-2222652 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT246447 WP_000534858.1 NZ_CP097882:2222126-2222365 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References