142456

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3281956..3282482 Replicon chromosome
Accession NZ_CP045741
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag GHR40_RS15735 Protein ID WP_000323025.1
Coordinates 3282195..3282482 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag GHR40_RS15730 Protein ID WP_000534858.1
Coordinates 3281956..3282195 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHR40_RS15670 3276983..3277750 - 768 Protein_3036 exonuclease -
GHR40_RS15675 3277843..3278034 - 192 WP_001083297.1 lysis protein YdfD -
GHR40_RS15680 3278031..3278219 - 189 WP_000854559.1 cell division inhibition protein DicB -
GHR40_RS15690 3278787..3279005 - 219 WP_001171942.1 hypothetical protein -
GHR40_RS15695 3279165..3279320 - 156 WP_000379575.1 DUF1391 family protein -
GHR40_RS15700 3279487..3279894 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
GHR40_RS15705 3279978..3280208 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
GHR40_RS15710 3280192..3280470 + 279 Protein_3043 hypothetical protein -
GHR40_RS15715 3280505..3280654 + 150 WP_011443592.1 hypothetical protein -
GHR40_RS15720 3281091..3281423 - 333 WP_001301033.1 protein FlxA -
GHR40_RS15725 3281626..3281931 - 306 WP_001326990.1 hypothetical protein -
GHR40_RS15730 3281956..3282195 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
GHR40_RS15735 3282195..3282482 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
GHR40_RS15740 3282554..3282709 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
GHR40_RS15745 3282926..3283177 + 252 WP_000980994.1 hypothetical protein -
GHR40_RS15750 3283244..3283522 + 279 WP_012304870.1 hypothetical protein -
GHR40_RS15755 3283524..3284573 + 1050 WP_001265198.1 DUF968 domain-containing protein -
GHR40_RS15760 3284587..3285339 + 753 WP_001047135.1 antitermination protein -
GHR40_RS15765 3285617..3285706 - 90 WP_120795389.1 hypothetical protein -
GHR40_RS15770 3285761..3285973 - 213 WP_000087756.1 cold shock-like protein CspF -
GHR40_RS15775 3286274..3286488 + 215 Protein_3056 cold shock-like protein CspB -
GHR40_RS15780 3287242..3287457 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3271084..3299468 28384


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T142456 WP_000323025.1 NZ_CP045741:3282195-3282482 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T142456 NZ_CP060703:1813254-1813841 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGTGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGTATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGTGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT142456 WP_000534858.1 NZ_CP045741:3281956-3282195 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT142456 NZ_CP060703:1813908-1814762 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGCTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGACAGCCTGGAAACCCAGACCCGCTGGCGTGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGTGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCATCCTTGACGATCTACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References