Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3281956..3282482 Replicon chromosome
Accession NZ_CP045741
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag GHR40_RS15735 Protein ID WP_000323025.1
Coordinates 3282195..3282482 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag GHR40_RS15730 Protein ID WP_000534858.1
Coordinates 3281956..3282195 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHR40_RS15670 3276983..3277750 - 768 Protein_3036 exonuclease -
GHR40_RS15675 3277843..3278034 - 192 WP_001083297.1 lysis protein YdfD -
GHR40_RS15680 3278031..3278219 - 189 WP_000854559.1 cell division inhibition protein DicB -
GHR40_RS15690 3278787..3279005 - 219 WP_001171942.1 hypothetical protein -
GHR40_RS15695 3279165..3279320 - 156 WP_000379575.1 DUF1391 family protein -
GHR40_RS15700 3279487..3279894 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
GHR40_RS15705 3279978..3280208 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
GHR40_RS15710 3280192..3280470 + 279 Protein_3043 hypothetical protein -
GHR40_RS15715 3280505..3280654 + 150 WP_011443592.1 hypothetical protein -
GHR40_RS15720 3281091..3281423 - 333 WP_001301033.1 protein FlxA -
GHR40_RS15725 3281626..3281931 - 306 WP_001326990.1 hypothetical protein -
GHR40_RS15730 3281956..3282195 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
GHR40_RS15735 3282195..3282482 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
GHR40_RS15740 3282554..3282709 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
GHR40_RS15745 3282926..3283177 + 252 WP_000980994.1 hypothetical protein -
GHR40_RS15750 3283244..3283522 + 279 WP_012304870.1 hypothetical protein -
GHR40_RS15755 3283524..3284573 + 1050 WP_001265198.1 DUF968 domain-containing protein -
GHR40_RS15760 3284587..3285339 + 753 WP_001047135.1 antitermination protein -
GHR40_RS15765 3285617..3285706 - 90 WP_120795389.1 hypothetical protein -
GHR40_RS15770 3285761..3285973 - 213 WP_000087756.1 cold shock-like protein CspF -
GHR40_RS15775 3286274..3286488 + 215 Protein_3056 cold shock-like protein CspB -
GHR40_RS15780 3287242..3287457 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3271084..3299468 28384


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T142456 WP_000323025.1 NZ_CP045741:3282195-3282482 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T142456 NZ_CP060703:1813254-1813841 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGTGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGTATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGTGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT142456 WP_000534858.1 NZ_CP045741:3281956-3282195 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT142456 NZ_CP060703:1813908-1814762 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGCTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGACAGCCTGGAAACCCAGACCCGCTGGCGTGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGTGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCATCCTTGACGATCTACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References