Detailed information of TA system TA285031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 4016426..4017863
• Replicon: chromosome
• Accession: NZ_HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100 strain K-12

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: BN896_RS19875
• Protein ID: WP_000155570.1
• Coordinates: 4016790..4017863 (-)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: BN896_RS19870
• Protein ID: WP_000461704.1
• Coordinates: 4016426..4016797 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_RS19835	4011627..4011941	+	315	WP_000065803.1	arsenate reductase	-
BN896_RS19840 (BN896_2394)	4012166..4012633	-	468	WP_000702495.1	hypothetical protein	-
BN896_RS19845 (BN896_2393)	4012657..4013100	-	444	WP_000824222.1	hypothetical protein	-
BN896_RS19850 (BN896_2392)	4013100..4013336	-	237	WP_001144029.1	hypothetical protein	-
BN896_RS19855 (BN896_2391)	4013377..4014078	-	702	WP_000189409.1	WYL domain-containing protein	-
BN896_RS19860 (BN896_2390)	4014295..4015116	-	822	WP_000197391.1	DUF945 domain-containing protein	-
BN896_RS19865 (BN896_2389)	4015208..4016071	-	864	WP_000787597.1	GTPase family protein	-
BN896_RS19870 (BN896_2388)	4016426..4016797	-	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
BN896_RS19875 (BN896_2387)	4016790..4017863	-	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
BN896_RS19880 (BN896_2386)	4018005..4018268	+	264	WP_000502050.1	DUF1524 domain-containing protein	-
BN896_RS19885 (BN896_2385)	4018628..4020244	+	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
BN896_RS19890 (BN896_2384)	4020241..4022430	+	2190	WP_000136402.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020085, TA020086 3996347..4028227 0 View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020085, TA020086 3996347..4028227 0 View NC_017625 Escherichia coli DH1 Genomic island TA027158, TA027159 1096328..1128208 0 View NZ_CP008801 Escherichia coli KLY Genomic island TA047514, TA047515 2732681..2774566 0.007 View NZ_CP009685 Escherichia coli str. K-12 substr. MG1655 strain K-12 Genomic island TA049432, TA049433 912721..944599 0 View NZ_CP010441 Escherichia coli K-12 strain K-12 MG1655 Genomic island TA052559, TA052560 3492901..3534786 0.007 View NZ_CP011113 Escherichia coli strain RR1 Genomic island TA053416, TA053417 2713006..2754891 0.008 View NZ_CP011124 Escherichia coli strain USML2 Genomic island TA053491, TA053492 2091893..2123773 0 View NZ_CP011320 Escherichia coli strain SQ37 Genomic island TA053855, TA053856 2753399..2795284 0.007 View NZ_CP012868 Escherichia coli str. K-12 substr. MG1655 strain K-12 Genomic island TA057296, TA057297 1854222..1886102 0 View NZ_CP012869 Escherichia coli strain K-12 substr. MG1655_TMP32XR1 Genomic island TA057327, TA057328 1854222..1886102 0 View NZ_CP012870 Escherichia coli strain K-12 substr. MG1655_TMP32XR2 Genomic island TA057358, TA057359 1854222..1886102 0 View NZ_CP013952 Escherichia coli strain HST04 Genomic island TA059659, TA059660 3102807..3134687 0 View NZ_CP014225 Escherichia coli str. K-12 substr. MG1655 strain K-12 Genomic island TA060382, TA060383 953359..995244 0.007 View NZ_CP014348 Escherichia coli str. K-12 substr. MG1655 strain JW5437-1 Genomic island TA060767, TA060768 2546240..2578120 0 View NZ_CP016018 Escherichia coli strain ER1821R Genomic island TA064127, TA064128 1096329..1128209 0 View NZ_CP018801 Escherichia coli strain tolC- Genomic island TA070541, TA070542 1098834..1130714 0 View NZ_CP026027 Escherichia coli strain LIM Genomic island TA092814, TA092815 2750373..2792258 0.007 View NZ_CP026343 Escherichia coli strain 9A Genomic island TA093915, TA093916 2758643..2788237 0.003 View NZ_CP026346 Escherichia coli strain 7FA Genomic island TA094044, TA094045 2756159..2788238 0.001 View NZ_CP031214 Escherichia coli strain C600 Genomic island TA108571, TA108572 1140616..1172496 0 View NZ_CP032667 Escherichia coli str. K-12 substr. MG1655 strain K-12 Genomic island TA112063, TA112064 1095941..1127820 0 View NZ_CP034595 Escherichia coli strain WCHEC035053S1G0 Genomic island TA116263, TA116264 953306..986524 0.001 View NZ_CP034953 Escherichia coli strain MT102 Genomic island TA117025, TA117026 1097362..1129242 0 View NZ_CP040667 Escherichia coli strain S17-1 Genomic island TA126442, TA126443 631404..664088 0.001 View NZ_CP042865 Escherichia coli strain ATCC BAA-196 Genomic island TA136295, TA136296 1097784..1129664 0 View NZ_CP042867 Escherichia coli strain ATCC BAA-196 Genomic island TA136326, TA136327 1097693..1129572 0 View NZ_CP043183 Escherichia coli O16:H48 strain PG20180172 Genomic island TA137398, TA137399 1100228..1132108 0 View NZ_CP043185 Escherichia coli O16:H48 strain PG20180171 Genomic island TA137439, TA137440 1482765..1524650 0.007 View NZ_CP043187 Escherichia coli O16:H48 strain PG20180170 Genomic island TA137466, TA137467 1100229..1132109 0 View NZ_CP043189 Escherichia coli O16:H48 strain PG20180175 Genomic island TA137497, TA137498 1100229..1132109 0 View NZ_CP043191 Escherichia coli O16:H48 strain PG20180173 Genomic island TA137529, TA137530 1100229..1132109 0 View NZ_CP043193 Escherichia coli O16:H48 strain PG20180063 Genomic island TA137559, TA137560 1100229..1132109 0 View NZ_CP043195 Escherichia coli O16:H48 strain PG20180062 Genomic island TA137590, TA137591 1388293..1420173 0 View NZ_CP043197 Escherichia coli O16:H48 strain PG20180061 Genomic island TA137630, TA137631 3079977..3111857 0 View NZ_CP043201 Escherichia coli O16:H48 strain PG20180059 Genomic island TA137699, TA137700 1100229..1132109 0 View NZ_CP043203 Escherichia coli O16:H48 strain PG20180058 Genomic island TA137732, TA137733 1100229..1132109 0 View NZ_CP043205 Escherichia coli O16:H48 strain PG20180054 Genomic island TA137765, TA137766 1100229..1132109 0 View NZ_CP043207 Escherichia coli O16:H48 strain PG20180053 Genomic island TA137798, TA137799 1100934..1130019 0.002 View NZ_CP043209 Escherichia coli O16:H48 strain PG20180052 Genomic island TA137831, TA137832 1100229..1132109 0 View NZ_CP043211 Escherichia coli O16:H48 strain PG20180050 Genomic island TA137864, TA137865 1385204..1417084 0 View NZ_CP045741 Escherichia coli strain DH5alpha Genomic island TA142444, TA142445 2145946..2178101 0.001 View NZ_CP046025 Escherichia coli strain C439 Genomic island TA143402, TA143403 1101565..1133445 0 View NZ_CP047127 Escherichia coli K-12 Genomic island TA145455, TA145456 2965953..2997820 0 View NZ_CP049112 Escherichia coli strain WF5-5-T2 Genomic island TA150556, TA150557 1099311..1131192 0 View NZ_CP053604 Escherichia coli strain NEB10-beta Genomic island TA157113, TA157114 2832052..2873937 0.007 View NZ_CP062240 Escherichia coli strain AML001_ev02 Genomic island TA173700, TA173701 2749518..2781597 0.001 View NZ_CP067994 Escherichia coli strain CV601 Genomic island TA186389, TA186390 1101523..1133403 0 View NZ_CP068283 Escherichia coli strain CY598 Genomic island TA187107, TA187108 2804676..2838091 0.002 View NZ_CP070482 Escherichia coli strain BTH101 Genomic island TA194161, TA194162 4575478..4607358 0 View NZ_CP070962 Escherichia coli strain K-12 J53 Genomic island TA194804, TA194805 772259..804137 0 View NZ_CP072054 Escherichia coli strain K1508 Genomic island TA197277, TA197278 3996347..4028227 0 View NZ_CP072986 Escherichia coli strain T159A Genomic island TA199401, TA199402 1101565..1133445 0 View NZ_CP076404 Escherichia coli strain K-12 substr. AB1157 Genomic island TA205641, TA205640 2702850..2744735 0.007 View NZ_CP076470 Escherichia coli strain DH5a Genomic island TA205734, TA205735 2963670..2995550 0 View NZ_CP079735 Escherichia coli strain GB2005 Genomic island TA209815, TA209816 1108899..1140779 0 View NZ_CP079736 Escherichia coli strain GB2005-delet-a-f Genomic island TA209842, TA209843 1108906..1139845 0.001 View NZ_CP083610 Escherichia coli strain EC2648-Vir1 Genomic island TA216837, TA216838 2579796..2611676 0 View NZ_CP083990 Escherichia coli strain EC2648-Vir2 Genomic island TA217882, TA217883 1098334..1130249 0.001 View NZ_CP084205 Escherichia coli strain EC2648-R1 Genomic island TA218202, TA218203 1098334..1128124 0.002 View NZ_CP084207 Escherichia coli strain EC2648-R1-1 Genomic island TA218243, TA218244 1907475..1939355 0 View NZ_CP084209 Escherichia coli strain EC2648-R2 Genomic island TA218274, TA218275 1098334..1130214 0 View NZ_CP087110 Escherichia coli strain PJ-T13 Genomic island TA224728, TA224729 953305..986523 0.001 View NZ_CP091065 Escherichia coli strain DOC14 Genomic island TA232615, TA232616 914319..946199 0 View NZ_CP093221 Escherichia coli strain ER2683 Genomic island TA237829, TA237830 2218271..2250151 0 View NZ_CP097881 Escherichia coli str. K-12 substr. MG1655 strain K-12 Genomic island TA246413, TA246414 1096295..1128175 0 View NZ_CP097882 Escherichia coli str. K-12 substr. MG1655 strain K-12 Genomic island TA246439, TA246440 1097496..1129376 0 View NZ_CP097883 Escherichia coli str. K-12 substr. MG1655 strain K-12 Genomic island TA246465, TA246466 1097496..1129376 0 View NZ_CP097884 Escherichia coli str. K-12 substr. MG1655 strain K-12 Genomic island TA246492, TA246493 1722629..1754509 0 View NZ_CP099588 Escherichia coli strain EMG2 Genomic island TA249137, TA249138 1098191..1130071 0 View NZ_CP099590 Escherichia coli strain WG1 Genomic island TA249168, TA249169 1103772..1135652 0 View NZ_CP104326 Escherichia coli strain THB42-F1 Genomic island TA257591, TA257592 1097656..1129536 0 View NZ_CP104328 Escherichia coli strain THB42-F2 Genomic island TA257622, TA257623 1098637..1130517 0 View NZ_CP104330 Escherichia coli strain THB42-F3 Genomic island TA257652, TA257653 1098334..1130214 0 View NZ_CP106994 Escherichia coli strain SY22A6 Genomic island TA260121, TA260122 1101759..1133639 0 View NZ_CP106995 Escherichia coli strain SY22B3 Genomic island TA260144, TA260145 1101759..1133639 0 View NZ_CP106996 Escherichia coli strain SY22D8 Genomic island TA260167, TA260168 1101759..1133639 0 View NZ_CP106997 Escherichia coli strain SY22B6 Genomic island TA260190, TA260191 1101759..1133639 0 View NZ_CP106998 Escherichia coli strain SY221 Genomic island TA260213, TA260214 1101759..1133639 0 View NZ_CP106999 Escherichia coli strain SY22A4 Genomic island TA260236, TA260237 1101759..1133639 0 View NZ_CP107000 Escherichia coli strain SY22B2 Genomic island TA260259, TA260260 1101759..1133639 0 View NZ_CP107001 Escherichia coli strain SY22B5 Genomic island TA260282, TA260283 1101759..1133639 0 View NZ_CP107002 Escherichia coli strain SY22C3 Genomic island TA260305, TA260306 1101759..1133639 0 View NZ_CP107003 Escherichia coli strain SY22B1 Genomic island TA260328, TA260329 1101759..1133639 0 View NZ_CP107004 Escherichia coli strain SY22A3 Genomic island TA260351, TA260352 1101759..1133639 0 View NZ_CP107005 Escherichia coli strain SY22A1 Genomic island TA260374, TA260375 1101759..1133639 0 View NZ_CP110814 Escherichia coli strain THB42-F4 Genomic island TA264299, TA264298 1098445..1130325 0 View NZ_CP110816 Escherichia coli strain THB42-F5 Genomic island TA264330, TA264331 1098334..1130214 0 View NZ_CP110859 Escherichia coli strain TSW4881-W1 Genomic island TA264391, TA264392 1101896..1133776 0 View NZ_CP110999 Escherichia coli strain J53-p133 Genomic island TA264719, TA264720 953306..986524 0.001 View NZ_CP111004 Escherichia coli strain J53-p131 Genomic island TA264748, TA264749 953306..986524 0.001 View NZ_CP111008 Escherichia coli strain J53-p130 Genomic island TA264776, TA264777 953306..986524 0.001 View NZ_CP114893 Escherichia coli strain CM13 Genomic island TA266953, TA266954 2323387..2360392 0.003 View NZ_CP114896 Escherichia coli strain CM93 Genomic island TA267028, TA267029 2212574..2254459 0.007 View NZ_CP117010 Escherichia coli strain MLI110 Genomic island TA269792, TA269793 1095150..1130681 0.004 View NZ_CP117962 Escherichia coli strain JM109 Genomic island TA272556, TA272557 3374655..3406535 0 View NZ_CP122316 Escherichia coli strain HT-946 Genomic island TA276659, TA276660 94764..126644 0 View NZ_CP122318 Escherichia coli strain HT873X1 Genomic island TA276721, TA276722 128178..160058 0 View NZ_LM993812 Escherichia coli strain K-12 substr. HMS174 Genomic island TA285406, TA285407 2708540..2740619 0.001	inside	Genomic island	-	-	3996347..4028227	31880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T285031 WP_000155570.1 NZ_HG738867:c4017863-4016790 [Escherichia coli str. K-12 substr. MC4100]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T285031 NZ_HG738867:c4017863-4016790 [Escherichia coli str. K-12 substr. MC4100]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT285031 WP_000461704.1 NZ_HG738867:c4016797-4016426 [Escherichia coli str. K-12 substr. MC4100]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT285031 NZ_HG738867:c4016797-4016426 [Escherichia coli str. K-12 substr. MC4100]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4