Detailed information of TA system TA052560

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3513857..3514524
• Replicon: chromosome
• Accession: NZ_CP010441
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: Q46953
• Locus tag: SH03_RS17365
• Protein ID: WP_001094400.1
• Coordinates: 3514195..3514524 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: P52141
• Locus tag: SH03_RS17360
• Protein ID: WP_000072690.1
• Coordinates: 3513857..3514174 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH03_RS17340	3508909..3509918	-	1010	Protein_3279	arsenic transporter	-
SH03_RS17345	3510060..3511763	+	1704	WP_000896263.1	hypothetical protein	-
SH03_RS24380	3512335..3512558	+	224	Protein_3281	DUF905 family protein	-
SH03_RS17350	3512661..3513119	+	459	WP_000211841.1	antirestriction protein	-
SH03_RS17355	3513128..3513610	+	483	WP_001407480.1	RadC family protein	-
SH03_RS24385	3513619..3513819	+	201	WP_001395452.1	DUF987 domain-containing protein	-
SH03_RS17360	3513857..3514174	+	318	WP_000072690.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
SH03_RS17365	3514195..3514524	+	330	WP_001094400.1	type IV toxin-antitoxin system toxin YpjF	Toxin
SH03_RS23000	3514888..3519468	-	4581	WP_010723187.1	adhesin-like autotransporter YpjA/EhaD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020085, TA020086 3996347..4028227 0.007 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285030, TA285031 3996347..4028227 0.007	inside	Genomic island	-	-	3492901..3534786	41885

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12308.21 Da
• Isoelectric Point: 7.2761

>T52560 WP_001094400.1 NZ_CP010441:3514195-3514524 [Escherichia coli K-12]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T52560 NZ_CP010441:3514195-3514524 [Escherichia coli K-12]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11737.27 Da
• Isoelectric Point: 6.4630

>AT52560 WP_000072690.1 NZ_CP010441:3513857-3514174 [Escherichia coli K-12]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT52560 NZ_CP010441:3513857-3514174 [Escherichia coli K-12]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A373F4I3

Antitoxin

• PDB: 2EA9
• PDB: 2JN7
• AlphaFold DB: P52141