285030

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 4005999..4006666 Replicon chromosome
Accession NZ_HG738867
Organism Escherichia coli str. K-12 substr. MC4100 strain K-12

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag BN896_RS19805 Protein ID WP_001094400.1
Coordinates 4005999..4006328 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag BN896_RS19810 Protein ID WP_000072690.1
Coordinates 4006349..4006666 (-) Length 106 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BN896_RS24840 (BN896_2401) 4001055..4005635 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
BN896_RS19805 (BN896_2400) 4005999..4006328 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
BN896_RS19810 (BN896_2399) 4006349..4006666 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BN896_RS24155 (BN896_2398) 4006704..4006904 - 201 WP_001395452.1 DUF987 domain-containing protein -
BN896_RS19815 (BN896_2397) 4006913..4007395 - 483 WP_001407480.1 RadC family protein -
BN896_RS19820 (BN896_2396) 4007404..4007862 - 459 WP_000211841.1 antirestriction protein -
BN896_RS24160 4007965..4008188 - 224 Protein_3754 DUF905 family protein -
BN896_RS19825 (BN896_2395) 4008760..4010463 - 1704 WP_000896263.1 hypothetical protein -
BN896_RS19830 4010605..4011614 + 1010 Protein_3756 arsenic transporter -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3996347..4028227 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-108)

Antitoxin

(5-104)


Sequences


Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T285030 WP_001094400.1 NZ_HG738867:c4006328-4005999 [Escherichia coli str. K-12 substr. MC4100]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T285030 NZ_HG738867:c4006328-4005999 [Escherichia coli str. K-12 substr. MC4100]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT285030 WP_000072690.1 NZ_HG738867:c4006666-4006349 [Escherichia coli str. K-12 substr. MC4100]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT285030 NZ_HG738867:c4006666-4006349 [Escherichia coli str. K-12 substr. MC4100]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A373F4I3


Antitoxin

Source ID Structure
AlphaFold DB P52141

References