Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2155885..2156552 Replicon chromosome
Accession NZ_CP045741
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag GHR40_RS10265 Protein ID WP_001094400.1
Coordinates 2155885..2156214 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag GHR40_RS10270 Protein ID WP_000072690.1
Coordinates 2156235..2156552 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHR40_RS10260 2150941..2155521 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
GHR40_RS10265 2155885..2156214 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
GHR40_RS10270 2156235..2156552 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GHR40_RS10275 2156590..2156790 - 201 WP_001395452.1 DUF987 domain-containing protein -
GHR40_RS10280 2156799..2157281 - 483 WP_001407480.1 RadC family protein -
GHR40_RS10285 2157290..2157748 - 459 WP_000211841.1 antirestriction protein -
GHR40_RS10290 2157851..2158074 - 224 Protein_1985 DUF905 family protein -
GHR40_RS10295 2158646..2160340 - 1695 Protein_1986 hypothetical protein -
GHR40_RS10300 2160482..2161491 + 1010 Protein_1987 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2145946..2178101 32155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T142444 WP_001094400.1 NZ_CP045741:c2156214-2155885 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T142444 NZ_CP060698:c2605337-2605035 [Amniculibacterium sp. G2-70]
ATGAATTACAAAATAAGCGTTGAAGCAAAGAATGACATTGAGAAAATTTGGCTTTACACTTTTGAGAATTGGTCGCTCAA
ACAAGCTGACCGCTATTTTGATTTGATAATGGACGAAGTTGAATACATTGCTGAAAACCCAAATTCTGGAAAGGATTATA
GTGATGTAAGAAAAGGATACTTGAGAAGTCGAATAAAATCACATTTCATTTTCTACAAAGTGAACAAAAAGCAAAATGAA
GTAGAAATTATTAGAGTTCTACACCAAAGCATGGATATTGAAAACAGACTCAATGACGAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT142444 WP_000072690.1 NZ_CP045741:c2156552-2156235 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT142444 NZ_CP060698:c2605585-2605334 [Amniculibacterium sp. G2-70]
ATGGCTAAAAATACATCGATTTTGTTGGGCGACTATTTTGACAACTTCATAAACCAACAAATTAAAAGTGGAAAATTCAC
TTCGGCAAGTGAAGTTGTACGAACAGCATTAAGAATGTTTGAATTTGAAGAATCTAAAAAAACGGAATTGATAAACGAAC
TGAAAAAAGGAGAAAAATCAGGCTTCGTTAAAGAATTTAACAGAGAAAACTTCCGAAAAGAACTTCATCAAAAACATCTT
GCTGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References