150557

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1119391..1120828 Replicon chromosome
Accession NZ_CP049112
Organism Escherichia coli strain WF5-5-T2

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag G6R06_RS05455 Protein ID WP_000155570.1
Coordinates 1119755..1120828 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag G6R06_RS05450 Protein ID WP_000461704.1
Coordinates 1119391..1119762 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6R06_RS05415 (1114591) 1114591..1114743 + 153 Protein_1056 arsenate reductase -
G6R06_RS05420 (1115130) 1115130..1115597 - 468 WP_000702495.1 protein YfjT -
G6R06_RS05425 (1115621) 1115621..1116064 - 444 WP_000824222.1 lipoprotein YfjS -
G6R06_RS05430 (1116064) 1116064..1116300 - 237 WP_001144029.1 protein YpjK -
G6R06_RS05435 (1116341) 1116341..1117042 - 702 WP_000189409.1 WYL domain-containing protein -
G6R06_RS05440 (1117259) 1117259..1118080 - 822 WP_000197391.1 DUF932 domain-containing protein -
G6R06_RS05445 (1118172) 1118172..1119035 - 864 WP_103119217.1 GTPase family protein -
G6R06_RS05450 (1119391) 1119391..1119762 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
G6R06_RS05455 (1119755) 1119755..1120828 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
G6R06_RS05460 (1120970) 1120970..1121233 + 264 WP_000502050.1 DUF1524 domain-containing protein -
G6R06_RS05465 (1121593) 1121593..1123209 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
G6R06_RS05470 (1123206) 1123206..1125395 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1099311..1131192 31881


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T150557 WP_000155570.1 NZ_CP049112:c1120828-1119755 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T150557 NZ_CP064794:c2061951-2061100 [Xanthomonas oryzae pv. oryzicola]
ATGAGCAACGTCGCCGCTTCCGTGCGCGCCCGCCTGCGGGGCGTCGCCAAGGCGCAGGGCGTGGATTTCAATCAGGTGCT
GGTGCGCTTCGCGCTGGAGCGCATCCTTTACCGCCTGAGCCAGTCCGAGCACGCGGATCGTTTCCTGCTCAAGGGCGCGC
TGCTGTTCACGCTCTGGTATGACATGCCGCACCGGGCCACGCGCGACGCCGACCTGCTGGGCTTCGGCGCGAGCGATCTG
GAGTCCGTGGCCCAGACCTTCCGCGACATCGCCAGCGTCGCCGTGGACGACGGCATCGTGTTCGACCCGGCCTCGGTCGT
CGCAGAAGAAATTCGCAAGGACGCCGGATACGCCGGGGCGCGCATCCTCATCGGCGGCGAGCTGGCCAAGGCGCGGTGCA
GGACGCAGATCGACGTTGGCTTCGGCGATGCGGTCACACCGGCTCCGGTGCACTCGGTCTATCCGGTGTTGCTGGAAGAT
CTGCCTGCGCCTCGCCTGCGCACCTATCCCGTCTACACCGTCATCGCGGAAAAGCTCCACGCCATCGCGCTTCTGGGCAT
GACCAACAGTCGGATGAAGGATTACCTCGACCTGTCGGTGCTGCTGGAACGCGAATCCCTGGACATCGATCTGCTGGCGC
AAGCGGTGAAGGCCACTTTCGAGCGACGCGGCATGGCCGTGCCCGCTGCTCTGCCGATCGGCATGACCGACGAGTTCGCG
CACGACACGTCTCGGCAGGCGCTGTGGCAGGCATTCCTCAAGAAGAACGAACTCGCCCCCGAACCCCTGGCTGCCATCGT
GGTTCGACTGCGGGCGGCCTTGGAACCAGCGTTGCACCGGGCTGCTCGATGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT150557 WP_000461704.1 NZ_CP049112:c1119762-1119391 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT150557 NZ_CP064794:c2062553-2061948 [Xanthomonas oryzae pv. oryzicola]
ATGCTTTCCGACACCCACACCCAGCGCGTCCTCGATCTGGCCAGCCAGAAAGGACTGCTGCGCGCCAGCAATCTGGACGC
CATCAATGCGCCCCGGGTCGTTCTCACGCGCCTGACCGCTGCTGGCGTTCTGGAAAGGGTCGGACGCGGGCTGTACCGCC
TGCCGGACGCGCAGGGATCGGAATTCGAGAGTCTCGCGGCCATCGCCACCAAGGTGCCACAAGCCGTGTTCTGCCTGCTG
ACTGCGCTGCAGTTCCACGAACTGACCACGCAACTGCCGCGCCAGATCTGGATCGCCATGCCGCGCGGCAGTCACGCGCC
CCGGATCGGCTATCCGCCGGTCAAGATGGTTCAGATGACCGGCGACGTCTACGCTGCAGGCATCGAGGAGCACCTGCGCG
ACGGCGTGAAGCTGCGCGTGTACGGTGCGGCCAAGACGGTCGTGGACTGCTTCAAGCACCGCAACAAGATCGGCCTGGAC
GTGGCGCTGGAGGCGCTGAAGGACGTGCGCGCCAAACGCAAGGCCACTGCGGACGACCTGTGGCGCTTTGCTACGGTCTG
CCGCGTGGCCAACGTGATGCGCCCCTATCTGGAGGCAGTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References