272557

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 3394734..3396171 Replicon chromosome
Accession NZ_CP117962
Organism Escherichia coli strain JM109

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag PT299_RS17690 Protein ID WP_000155570.1
Coordinates 3395098..3396171 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag PT299_RS17685 Protein ID WP_000461704.1
Coordinates 3394734..3395105 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT299_RS17650 (3389935) 3389935..3390087 + 153 Protein_3194 arsenate reductase -
PT299_RS17655 (3390474) 3390474..3390941 - 468 WP_000702495.1 protein YfjT -
PT299_RS17660 (3390965) 3390965..3391408 - 444 WP_000824222.1 lipoprotein YfjS -
PT299_RS17665 (3391408) 3391408..3391644 - 237 WP_001144029.1 protein YpjK -
PT299_RS17670 (3391685) 3391685..3392386 - 702 WP_000189409.1 WYL domain-containing protein -
PT299_RS17675 (3392603) 3392603..3393424 - 822 WP_000197391.1 DUF932 domain-containing protein -
PT299_RS17680 (3393516) 3393516..3394379 - 864 WP_000787597.1 GTPase family protein -
PT299_RS17685 (3394734) 3394734..3395105 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
PT299_RS17690 (3395098) 3395098..3396171 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
PT299_RS17695 (3396313) 3396313..3396576 + 264 WP_000502050.1 DUF1524 domain-containing protein -
PT299_RS17700 (3396936) 3396936..3398552 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
PT299_RS17705 (3398549) 3398549..3400738 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3374655..3406535 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T272557 WP_000155570.1 NZ_CP117962:c3396171-3395098 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT272557 WP_000461704.1 NZ_CP117962:c3395105-3394734 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References