Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | rnlAB/RnlA-RnlB |
| Location | 2841811..2843248 | Replicon | chromosome |
| Accession | NZ_CP053604 | ||
| Organism | Escherichia coli strain NEB10-beta | ||
Toxin (Protein)
| Gene name | rnlA | Uniprot ID | P52129 |
| Locus tag | HO394_RS13825 | Protein ID | WP_000155570.1 |
| Coordinates | 2841811..2842884 (+) | Length | 358 a.a. |
Antitoxin (Protein)
| Gene name | rnlB | Uniprot ID | P52130 |
| Locus tag | HO394_RS13830 | Protein ID | WP_000461704.1 |
| Coordinates | 2842877..2843248 (+) | Length | 124 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HO394_RS13810 | 2837244..2839433 | - | 2190 | WP_000136402.1 | DEAD/DEAH box helicase | - |
| HO394_RS13815 | 2839430..2841046 | - | 1617 | WP_000445380.1 | anti-bacteriophage protein AbpA | - |
| HO394_RS13820 | 2841406..2841669 | - | 264 | WP_000502050.1 | DUF1524 domain-containing protein | - |
| HO394_RS13825 | 2841811..2842884 | + | 1074 | WP_000155570.1 | type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA | Toxin |
| HO394_RS13830 | 2842877..2843248 | + | 372 | WP_000461704.1 | type II toxin-antitoxin system antitoxin RnlB | Antitoxin |
| HO394_RS13835 | 2843603..2844466 | + | 864 | WP_000787597.1 | GTPase family protein | - |
| HO394_RS13840 | 2844558..2845379 | + | 822 | WP_000197391.1 | DUF945 domain-containing protein | - |
| HO394_RS13845 | 2845596..2846297 | + | 702 | WP_000189409.1 | WYL domain-containing protein | - |
| HO394_RS13850 | 2846338..2846574 | + | 237 | WP_001144029.1 | hypothetical protein | - |
| HO394_RS13855 | 2846574..2847017 | + | 444 | WP_000824222.1 | hypothetical protein | - |
| HO394_RS13860 | 2847041..2847508 | + | 468 | WP_000702495.1 | hypothetical protein | - |
| HO394_RS13865 | 2847733..2848047 | - | 315 | WP_000065803.1 | arsenate reductase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Genomic island | - | - | 2832052..2873937 | 41885 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 358 a.a. Molecular weight: 40061.13 Da Isoelectric Point: 6.8293
>T157113 WP_000155570.1 NZ_CP053604:2841811-2842884 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV
Download Length: 1074 bp
>T157113 NZ_CP069076:2855592-2856005 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA
Antitoxin
Download Length: 124 a.a. Molecular weight: 13664.69 Da Isoelectric Point: 4.2391
>AT157113 WP_000461704.1 NZ_CP053604:2842877-2843248 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF
Download Length: 372 bp
>AT157113 NZ_CP069076:2856044-2856301 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|