145456

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2986021..2987458 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag GRF62_RS14285 Protein ID WP_000155570.1
Coordinates 2986385..2987458 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag GRF62_RS14280 Protein ID WP_000461704.1
Coordinates 2986021..2986392 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS14245 2981222..2981536 + 315 WP_000065803.1 arsenate reductase -
GRF62_RS14250 2981761..2982228 - 468 WP_000702495.1 hypothetical protein -
GRF62_RS14255 2982252..2982695 - 444 WP_000824222.1 hypothetical protein -
GRF62_RS14260 2982695..2982931 - 237 WP_001144029.1 hypothetical protein -
GRF62_RS14265 2982972..2983673 - 702 WP_000189409.1 WYL domain-containing protein -
GRF62_RS14270 2983890..2984711 - 822 WP_000197391.1 DUF945 domain-containing protein -
GRF62_RS14275 2984803..2985666 - 864 WP_000787597.1 GTPase family protein -
GRF62_RS14280 2986021..2986392 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
GRF62_RS14285 2986385..2987458 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
GRF62_RS14290 2987600..2987863 + 264 WP_000502050.1 DUF1524 domain-containing protein -
GRF62_RS14295 2988223..2989839 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
GRF62_RS14300 2989836..2992025 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2965953..2997820 31867


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T145456 WP_000155570.1 NZ_CP047127:c2987458-2986385 [Escherichia coli K-12]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T145456 NZ_CP062386:2742183-2742434 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT145456 WP_000461704.1 NZ_CP047127:c2986392-2986021 [Escherichia coli K-12]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT145456 NZ_CP062386:2742424-2742597 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGATGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References