Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | rnlAB/RnlA-RnlB |
| Location | 1120308..1121745 | Replicon | chromosome |
| Accession | NZ_CP043209 | ||
| Organism | Escherichia coli O16:H48 strain PG20180052 | ||
Toxin (Protein)
| Gene name | rnlA | Uniprot ID | P52129 |
| Locus tag | FYA06_RS05425 | Protein ID | WP_000155570.1 |
| Coordinates | 1120672..1121745 (-) | Length | 358 a.a. |
Antitoxin (Protein)
| Gene name | rnlB | Uniprot ID | P52130 |
| Locus tag | FYA06_RS05420 | Protein ID | WP_000461704.1 |
| Coordinates | 1120308..1120679 (-) | Length | 124 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FYA06_RS05385 | 1115509..1115823 | + | 315 | WP_000065803.1 | arsenate reductase | - |
| FYA06_RS05390 | 1116048..1116515 | - | 468 | WP_000702495.1 | hypothetical protein | - |
| FYA06_RS05395 | 1116539..1116982 | - | 444 | WP_000824222.1 | hypothetical protein | - |
| FYA06_RS05400 | 1116982..1117218 | - | 237 | WP_001144029.1 | hypothetical protein | - |
| FYA06_RS05405 | 1117259..1117960 | - | 702 | WP_000189409.1 | WYL domain-containing protein | - |
| FYA06_RS05410 | 1118177..1118998 | - | 822 | WP_000197391.1 | DUF945 domain-containing protein | - |
| FYA06_RS05415 | 1119090..1119953 | - | 864 | WP_000787597.1 | GTPase family protein | - |
| FYA06_RS05420 | 1120308..1120679 | - | 372 | WP_000461704.1 | type II toxin-antitoxin system antitoxin RnlB | Antitoxin |
| FYA06_RS05425 | 1120672..1121745 | - | 1074 | WP_000155570.1 | type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA | Toxin |
| FYA06_RS05430 | 1121887..1122150 | + | 264 | WP_000502050.1 | DUF1524 domain-containing protein | - |
| FYA06_RS05435 | 1122510..1124126 | + | 1617 | WP_000445380.1 | anti-bacteriophage protein AbpA | - |
| FYA06_RS05440 | 1124123..1126312 | + | 2190 | WP_000136402.1 | DEAD/DEAH box helicase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Genomic island | - | - | 1100229..1132109 | 31880 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 358 a.a. Molecular weight: 40061.13 Da Isoelectric Point: 6.8293
>T137832 WP_000155570.1 NZ_CP043209:c1121745-1120672 [Escherichia coli O16:H48]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV
Download Length: 1074 bp
>T137832 NZ_CP057066:c4579020-4578736 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA
Antitoxin
Download Length: 124 a.a. Molecular weight: 13664.69 Da Isoelectric Point: 4.2391
>AT137832 WP_000461704.1 NZ_CP043209:c1120679-1120308 [Escherichia coli O16:H48]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF
Download Length: 372 bp
>AT137832 NZ_CP043209:c1120679-1120308 [Escherichia coli O16:H48]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|