Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1116374..1117811 Replicon chromosome
Accession NZ_CP097881
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag NAG69_RS05390 Protein ID WP_000155570.1
Coordinates 1116738..1117811 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag NAG69_RS05385 Protein ID WP_000461704.1
Coordinates 1116374..1116745 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG69_RS05350 (1111575) 1111575..1111727 + 153 Protein_1043 arsenate reductase -
NAG69_RS05355 (1112114) 1112114..1112581 - 468 WP_000702495.1 protein YfjT -
NAG69_RS05360 (1112605) 1112605..1113048 - 444 WP_000824222.1 lipoprotein YfjS -
NAG69_RS05365 (1113048) 1113048..1113284 - 237 WP_001144029.1 protein YpjK -
NAG69_RS05370 (1113325) 1113325..1114026 - 702 WP_000189409.1 WYL domain-containing protein -
NAG69_RS05375 (1114243) 1114243..1115064 - 822 WP_000197391.1 DUF932 domain-containing protein -
NAG69_RS05380 (1115156) 1115156..1116019 - 864 WP_000787597.1 GTPase family protein -
NAG69_RS05385 (1116374) 1116374..1116745 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
NAG69_RS05390 (1116738) 1116738..1117811 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
NAG69_RS05395 (1117953) 1117953..1118216 + 264 WP_000502050.1 DUF1524 domain-containing protein -
NAG69_RS05400 (1118576) 1118576..1120192 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
NAG69_RS05405 (1120189) 1120189..1122378 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1096295..1128175 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T246414 WP_000155570.1 NZ_CP097881:c1117811-1116738 [Escherichia coli str. K-12 substr. MG1655]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT246414 WP_000461704.1 NZ_CP097881:c1116745-1116374 [Escherichia coli str. K-12 substr. MG1655]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References