Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1121644..1123081 Replicon chromosome
Accession NZ_CP046025
Organism Escherichia coli strain C439

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag GJH08_RS05380 Protein ID WP_000155570.1
Coordinates 1122008..1123081 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag GJH08_RS05375 Protein ID WP_000461704.1
Coordinates 1121644..1122015 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJH08_RS05340 1116845..1117159 + 315 WP_000065803.1 arsenate reductase -
GJH08_RS05345 1117384..1117851 - 468 WP_000702495.1 hypothetical protein -
GJH08_RS05350 1117875..1118318 - 444 WP_000824222.1 hypothetical protein -
GJH08_RS05355 1118318..1118554 - 237 WP_001144029.1 hypothetical protein -
GJH08_RS05360 1118595..1119296 - 702 WP_000189409.1 WYL domain-containing protein -
GJH08_RS05365 1119513..1120334 - 822 WP_000197391.1 DUF945 domain-containing protein -
GJH08_RS05370 1120426..1121289 - 864 WP_000787597.1 GTPase family protein -
GJH08_RS05375 1121644..1122015 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
GJH08_RS05380 1122008..1123081 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
GJH08_RS05385 1123223..1123486 + 264 WP_000502050.1 DUF1524 domain-containing protein -
GJH08_RS05390 1123846..1125462 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
GJH08_RS05395 1125459..1127648 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1101565..1133445 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T143403 WP_000155570.1 NZ_CP046025:c1123081-1122008 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T143403 NZ_CP061081:777599-777883 [Marinomonas arctica]
CTGACTTATAAACTCGAATTCAAAAAGAGCGCCTTGAAGGAGTGGCGAAAACTTGGTGCTACGCTGCAACAGCAATTTAA
GAAAAAACTGATCGAACGTTTAGATCATCCACATGTTCCTGCTTCAAAGCTTTCAGGGGCTGACAACCTTTACAAAATCA
AATTACGCCAATCAGGTTATCGCCTTGTCTACGAAGTACAAGACGATGTCGTTATTGTCACTGTGTTGGCAGTAGGAAAG
CGGGAGCGTAATGAGGTTTATAAAAATGCTATTAAACGGGTTTAG

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT143403 WP_000461704.1 NZ_CP046025:c1122015-1121644 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT143403 NZ_CP061081:777361-777609 [Marinomonas arctica]
ATGACTTCTAGAATCCTTTCCGACGTGGCTGCAAGCATTACTGAGTTTAAAGCCAACCCGATGAAAGTCGCTGCAAGCGC
ACATGGTAAAGCGGTTGCAGTATTAAATCGCAATGAACCTGCGTTTTACTGTGTCCCTGCTGAAGCTTATGAAGCCTTAA
TGGATAAATTGGAAGATATGGAACTGATCTCACTCGCGAAGGAACGCGTCAATGAAGAAAGTATCTCAGTCAGCATCGCT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References