Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1397945..1398612 Replicon chromosome
Accession NZ_CP043195
Organism Escherichia coli O16:H48 strain PG20180062

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag FYA13_RS06680 Protein ID WP_001094400.1
Coordinates 1397945..1398274 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag FYA13_RS06685 Protein ID WP_000072690.1
Coordinates 1398295..1398612 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA13_RS06675 1393001..1397581 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
FYA13_RS06680 1397945..1398274 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
FYA13_RS06685 1398295..1398612 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FYA13_RS06690 1398650..1398850 - 201 WP_001395452.1 DUF987 domain-containing protein -
FYA13_RS06695 1398859..1399341 - 483 WP_001407480.1 RadC family protein -
FYA13_RS06700 1399350..1399808 - 459 WP_000211841.1 antirestriction protein -
FYA13_RS06705 1399911..1400134 - 224 Protein_1291 DUF905 domain-containing protein -
FYA13_RS06710 1400706..1402409 - 1704 WP_000896263.1 hypothetical protein -
FYA13_RS06715 1402551..1403560 + 1010 Protein_1293 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1388293..1420173 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T137590 WP_001094400.1 NZ_CP043195:c1398274-1397945 [Escherichia coli O16:H48]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T137590 NZ_CP056835:95608-96138 [Citrobacter sp. RHBSTW-00127]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT137590 WP_000072690.1 NZ_CP043195:c1398612-1398295 [Escherichia coli O16:H48]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT137590 NZ_CP043195:c1398612-1398295 [Escherichia coli O16:H48]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References