112063

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1105592..1106259 Replicon chromosome
Accession NZ_CP032667
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag D8B36_RS05415 Protein ID WP_001094400.1
Coordinates 1105592..1105921 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag D8B36_RS05420 Protein ID WP_000072690.1
Coordinates 1105942..1106259 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8B36_RS05410 1100648..1105228 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
D8B36_RS05415 1105592..1105921 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
D8B36_RS05420 1105942..1106259 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
D8B36_RS05425 1106297..1106497 - 201 WP_001395452.1 DUF987 domain-containing protein -
D8B36_RS05430 1106506..1106988 - 483 WP_001407480.1 RadC family protein -
D8B36_RS05435 1106997..1107455 - 459 WP_000211841.1 antirestriction protein -
D8B36_RS05440 1107558..1107781 - 224 Protein_1039 DUF905 domain-containing protein -
D8B36_RS05445 1108353..1110056 - 1704 WP_000896263.1 hypothetical protein -
D8B36_RS05450 1110198..1111207 + 1010 Protein_1041 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1095941..1127820 31879


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T112063 WP_001094400.1 NZ_CP032667:c1105921-1105592 [Escherichia coli str. K-12 substr. MG1655]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T112063 NZ_CP032667:c1105921-1105592 [Escherichia coli str. K-12 substr. MG1655]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT112063 WP_000072690.1 NZ_CP032667:c1106259-1105942 [Escherichia coli str. K-12 substr. MG1655]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT112063 NZ_CP032667:c1106259-1105942 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
AlphaFold DB P52141

References