Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1105980..1106647 Replicon chromosome
Accession NC_017625
Organism Escherichia coli DH1

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag ECDH1_RS05460 Protein ID WP_001094400.1
Coordinates 1105980..1106309 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag ECDH1_RS05465 Protein ID WP_000072690.1
Coordinates 1106330..1106647 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH1_RS25020 1101036..1105616 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
ECDH1_RS05460 1105980..1106309 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
ECDH1_RS05465 1106330..1106647 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECDH1_RS23485 1106685..1106885 - 201 WP_001395452.1 DUF987 domain-containing protein -
ECDH1_RS05470 1106894..1107376 - 483 WP_001407480.1 RadC family protein -
ECDH1_RS05475 1107385..1107843 - 459 WP_000211841.1 antirestriction protein -
ECDH1_RS23490 1107946..1108169 - 224 Protein_1032 DUF905 family protein -
ECDH1_RS05480 1108741..1110444 - 1704 WP_000896263.1 hypothetical protein -
ECDH1_RS05485 1110586..1111595 + 1010 Protein_1034 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1096328..1128208 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T27158 WP_001094400.1 NC_017625:c1106309-1105980 [Escherichia coli DH1]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T27158 NC_017625:c1106309-1105980 [Escherichia coli DH1]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT27158 WP_000072690.1 NC_017625:c1106647-1106330 [Escherichia coli DH1]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT27158 NC_017625:c1106647-1106330 [Escherichia coli DH1]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References