Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2555892..2556559 Replicon chromosome
Accession NZ_CP014348
Organism Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag AWN69_RS12600 Protein ID WP_001094400.1
Coordinates 2555892..2556221 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag AWN69_RS12605 Protein ID WP_000072690.1
Coordinates 2556242..2556559 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWN69_RS25240 2550948..2555528 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
AWN69_RS12600 2555892..2556221 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
AWN69_RS12605 2556242..2556559 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AWN69_RS24215 2556597..2556797 - 201 WP_001395452.1 DUF987 domain-containing protein -
AWN69_RS12610 2556806..2557288 - 483 WP_001407480.1 RadC family protein -
AWN69_RS12615 2557297..2557755 - 459 WP_000211841.1 antirestriction protein -
AWN69_RS24220 2557858..2558081 - 224 Protein_2391 DUF905 family protein -
AWN69_RS12620 2558653..2560356 - 1704 WP_000896263.1 hypothetical protein -
AWN69_RS12625 2560498..2561507 + 1010 Protein_2393 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2546240..2578120 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T60767 WP_001094400.1 NZ_CP014348:c2556221-2555892 [Escherichia coli str. K-12 substr. MG1655]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T60767 NZ_CP014348:c2556221-2555892 [Escherichia coli str. K-12 substr. MG1655]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT60767 WP_000072690.1 NZ_CP014348:c2556559-2556242 [Escherichia coli str. K-12 substr. MG1655]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT60767 NZ_CP014348:c2556559-2556242 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References