249169

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1123851..1125288 Replicon chromosome
Accession NZ_CP099590
Organism Escherichia coli strain WG1

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag NHF37_RS05415 Protein ID WP_000155570.1
Coordinates 1124215..1125288 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag NHF37_RS05410 Protein ID WP_000461704.1
Coordinates 1123851..1124222 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF37_RS05375 (1119052) 1119052..1119204 + 153 Protein_1058 arsenate reductase -
NHF37_RS05380 (1119591) 1119591..1120058 - 468 WP_000702495.1 protein YfjT -
NHF37_RS05385 (1120082) 1120082..1120525 - 444 WP_000824222.1 lipoprotein YfjS -
NHF37_RS05390 (1120525) 1120525..1120761 - 237 WP_001144029.1 protein YpjK -
NHF37_RS05395 (1120802) 1120802..1121503 - 702 WP_000189409.1 WYL domain-containing protein -
NHF37_RS05400 (1121720) 1121720..1122541 - 822 WP_000197391.1 DUF932 domain-containing protein -
NHF37_RS05405 (1122633) 1122633..1123496 - 864 WP_000787597.1 GTPase family protein -
NHF37_RS05410 (1123851) 1123851..1124222 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
NHF37_RS05415 (1124215) 1124215..1125288 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
NHF37_RS05420 (1125430) 1125430..1125693 + 264 WP_000502050.1 DUF1524 domain-containing protein -
NHF37_RS05425 (1126053) 1126053..1127669 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
NHF37_RS05430 (1127666) 1127666..1129855 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1103772..1135652 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T249169 WP_000155570.1 NZ_CP099590:c1125288-1124215 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT249169 WP_000461704.1 NZ_CP099590:c1124222-1123851 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References