216838

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2599875..2601312 Replicon chromosome
Accession NZ_CP083610
Organism Escherichia coli strain EC2648-Vir1

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag LAJ48_RS12540 Protein ID WP_000155570.1
Coordinates 2600239..2601312 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag LAJ48_RS12535 Protein ID WP_000461704.1
Coordinates 2599875..2600246 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAJ48_RS12500 (2595076) 2595076..2595228 + 153 Protein_2439 arsenate reductase -
LAJ48_RS12505 (2595615) 2595615..2596082 - 468 WP_000702495.1 protein YfjT -
LAJ48_RS12510 (2596106) 2596106..2596549 - 444 WP_000824222.1 lipoprotein YfjS -
LAJ48_RS12515 (2596549) 2596549..2596785 - 237 WP_001144029.1 protein YpjK -
LAJ48_RS12520 (2596826) 2596826..2597527 - 702 WP_000189409.1 WYL domain-containing protein -
LAJ48_RS12525 (2597744) 2597744..2598565 - 822 WP_000197391.1 DUF932 domain-containing protein -
LAJ48_RS12530 (2598657) 2598657..2599520 - 864 WP_000787597.1 GTPase family protein -
LAJ48_RS12535 (2599875) 2599875..2600246 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
LAJ48_RS12540 (2600239) 2600239..2601312 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
LAJ48_RS12545 (2601454) 2601454..2601717 + 264 WP_000502050.1 DUF1524 domain-containing protein -
LAJ48_RS12550 (2602077) 2602077..2603693 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
LAJ48_RS12555 (2603690) 2603690..2605879 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2579796..2611676 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T216838 WP_000155570.1 NZ_CP083610:c2601312-2600239 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T216838 NZ_CP113493:c837432-837055 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGCCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATACGGTGAACTTTCTCGTTGAAAAATACGCACTGATACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAACATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT216838 WP_000461704.1 NZ_CP083610:c2600246-2599875 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT216838 NZ_CP113493:c837890-837522 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References