217883

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1118448..1119885 Replicon chromosome
Accession NZ_CP083990
Organism Escherichia coli strain EC2648-Vir2

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag LCH23_RS05400 Protein ID WP_000155570.1
Coordinates 1118812..1119885 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag LCH23_RS05395 Protein ID WP_000461704.1
Coordinates 1118448..1118819 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH23_RS05360 (1113649) 1113649..1113801 + 153 Protein_1053 arsenate reductase -
LCH23_RS05365 (1114188) 1114188..1114655 - 468 WP_000702495.1 protein YfjT -
LCH23_RS05370 (1114679) 1114679..1115122 - 444 WP_000824222.1 lipoprotein YfjS -
LCH23_RS05375 (1115122) 1115122..1115358 - 237 WP_001144029.1 protein YpjK -
LCH23_RS05380 (1115399) 1115399..1116100 - 702 WP_000189409.1 WYL domain-containing protein -
LCH23_RS05385 (1116317) 1116317..1117138 - 822 WP_000197391.1 DUF932 domain-containing protein -
LCH23_RS05390 (1117230) 1117230..1118093 - 864 WP_000787597.1 GTPase family protein -
LCH23_RS05395 (1118448) 1118448..1118819 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
LCH23_RS05400 (1118812) 1118812..1119885 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
LCH23_RS05405 (1120027) 1120027..1120290 + 264 WP_000502050.1 DUF1524 domain-containing protein -
LCH23_RS05410 (1120650) 1120650..1122266 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
LCH23_RS05415 (1122263) 1122263..1124452 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1098334..1130249 31915


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T217883 WP_000155570.1 NZ_CP083990:c1119885-1118812 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T217883 NZ_CP114893:c653547-653197 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT217883 WP_000461704.1 NZ_CP083990:c1118819-1118448 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT217883 NZ_CP114893:c653792-653541 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References