145455

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2975601..2976268 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag GRF62_RS14205 Protein ID WP_001094400.1
Coordinates 2975601..2975930 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag GRF62_RS14210 Protein ID WP_000072690.1
Coordinates 2975951..2976268 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS14200 2970657..2975237 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
GRF62_RS14205 2975601..2975930 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
GRF62_RS14210 2975951..2976268 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GRF62_RS14215 2976306..2976506 - 201 WP_001395452.1 DUF987 domain-containing protein -
GRF62_RS14220 2976515..2976997 - 483 WP_001407480.1 RadC family protein -
GRF62_RS14225 2977006..2977464 - 459 WP_000211841.1 antirestriction protein -
GRF62_RS14230 2977567..2977790 - 224 Protein_2756 DUF905 family protein -
GRF62_RS14235 2978362..2980058 - 1697 Protein_2757 hypothetical protein -
GRF62_RS14240 2980200..2981209 + 1010 Protein_2758 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2965953..2997820 31867


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T145455 WP_001094400.1 NZ_CP047127:c2975930-2975601 [Escherichia coli K-12]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T145455 NZ_CP062386:2670811-2671173 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT145455 WP_000072690.1 NZ_CP047127:c2976268-2975951 [Escherichia coli K-12]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT145455 NZ_CP062386:2670644-2670814 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
AlphaFold DB P52141

References