269793

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1118880..1120317 Replicon chromosome
Accession NZ_CP117010
Organism Escherichia coli strain MLI110

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag NL413_RS06120 Protein ID WP_000155570.1
Coordinates 1119244..1120317 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag NL413_RS06115 Protein ID WP_000461704.1
Coordinates 1118880..1119251 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL413_RS06090 (1115111) 1115111..1115554 - 444 WP_000824222.1 lipoprotein YfjS -
NL413_RS06095 (1115554) 1115554..1115790 - 237 WP_001144029.1 protein YpjK -
NL413_RS06100 (1115831) 1115831..1116532 - 702 WP_000189409.1 WYL domain-containing protein -
NL413_RS06105 (1116749) 1116749..1117570 - 822 WP_000197391.1 DUF932 domain-containing protein -
NL413_RS06110 (1117662) 1117662..1118525 - 864 WP_000787597.1 GTPase family protein -
NL413_RS06115 (1118880) 1118880..1119251 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
NL413_RS06120 (1119244) 1119244..1120317 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
NL413_RS06125 (1120459) 1120459..1120722 + 264 WP_000502050.1 DUF1524 domain-containing protein -
NL413_RS06130 (1121082) 1121082..1122698 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
NL413_RS06135 (1122695) 1122695..1124884 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1095150..1130681 35531
- inside Prophage - luxS 1051699..1130076 78377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T269793 WP_000155570.1 NZ_CP117010:c1120317-1119244 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT269793 WP_000461704.1 NZ_CP117010:c1119251-1118880 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References