276660

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 114843..116280 Replicon chromosome
Accession NZ_CP122316
Organism Escherichia coli strain HT-946

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag QCG48_RS00620 Protein ID WP_000155570.1
Coordinates 115207..116280 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag QCG48_RS00615 Protein ID WP_000461704.1
Coordinates 114843..115214 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCG48_RS00580 (110044) 110044..110196 + 153 Protein_109 arsenate reductase -
QCG48_RS00585 (110583) 110583..111050 - 468 WP_000702495.1 protein YfjT -
QCG48_RS00590 (111074) 111074..111517 - 444 WP_000824222.1 lipoprotein YfjS -
QCG48_RS00595 (111517) 111517..111753 - 237 WP_001144029.1 protein YpjK -
QCG48_RS00600 (111794) 111794..112495 - 702 WP_000189409.1 WYL domain-containing protein -
QCG48_RS00605 (112712) 112712..113533 - 822 WP_000197391.1 DUF932 domain-containing protein -
QCG48_RS00610 (113625) 113625..114488 - 864 WP_000787597.1 GTPase family protein -
QCG48_RS00615 (114843) 114843..115214 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
QCG48_RS00620 (115207) 115207..116280 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
QCG48_RS00625 (116422) 116422..116685 + 264 WP_000502050.1 DUF1524 domain-containing protein -
QCG48_RS00630 (117045) 117045..118661 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
QCG48_RS00635 (118658) 118658..120847 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 94764..126644 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T276660 WP_000155570.1 NZ_CP122316:c116280-115207 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT276660 WP_000461704.1 NZ_CP122316:c115214-114843 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References