Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1109880..1110547 Replicon chromosome
Accession NZ_CP043183
Organism Escherichia coli O16:H48 strain PG20180172

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag FYA19_RS05345 Protein ID WP_001094400.1
Coordinates 1109880..1110209 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag FYA19_RS05350 Protein ID WP_000072690.1
Coordinates 1110230..1110547 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA19_RS05340 1104936..1109516 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
FYA19_RS05345 1109880..1110209 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
FYA19_RS05350 1110230..1110547 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FYA19_RS05355 1110585..1110785 - 201 WP_001395452.1 DUF987 domain-containing protein -
FYA19_RS05360 1110794..1111276 - 483 WP_001407480.1 RadC family protein -
FYA19_RS05365 1111285..1111743 - 459 WP_000211841.1 antirestriction protein -
FYA19_RS05370 1111846..1112069 - 224 Protein_1044 DUF905 domain-containing protein -
FYA19_RS05375 1112641..1114344 - 1704 WP_000896263.1 hypothetical protein -
FYA19_RS05380 1114486..1115495 + 1010 Protein_1046 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1100228..1132108 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T137398 WP_001094400.1 NZ_CP043183:c1110209-1109880 [Escherichia coli O16:H48]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T137398 NZ_CP056717:c3073531-3073428 [Citrobacter sp. RHBSTW-00599]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT137398 WP_000072690.1 NZ_CP043183:c1110547-1110230 [Escherichia coli O16:H48]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT137398 NZ_CP056717:3073422-3073566 [Citrobacter sp. RHBSTW-00599]
ACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References