Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 3384307..3384974 Replicon chromosome
Accession NZ_CP117962
Organism Escherichia coli strain JM109

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag PT299_RS17610 Protein ID WP_001094400.1
Coordinates 3384307..3384636 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag PT299_RS17615 Protein ID WP_000072690.1
Coordinates 3384657..3384974 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT299_RS17605 (3379363) 3379363..3383943 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
PT299_RS17610 (3384307) 3384307..3384636 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
PT299_RS17615 (3384657) 3384657..3384974 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
PT299_RS17620 (3385012) 3385012..3385212 - 201 WP_001395452.1 DUF987 domain-containing protein -
PT299_RS17625 (3385221) 3385221..3385703 - 483 WP_001407480.1 RadC family protein -
PT299_RS17630 (3385712) 3385712..3386170 - 459 WP_000211841.1 antirestriction protein -
PT299_RS17635 (3386273) 3386273..3386457 - 185 Protein_3191 DUF905 family protein -
PT299_RS17640 (3387068) 3387068..3388771 - 1704 WP_000896263.1 protein YfjW -
PT299_RS17645 (3388913) 3388913..3389922 + 1010 Protein_3193 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3374655..3406535 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T272556 WP_001094400.1 NZ_CP117962:c3384636-3384307 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT272556 WP_000072690.1 NZ_CP117962:c3384974-3384657 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References