194162

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 4595557..4596994 Replicon chromosome
Accession NZ_CP070482
Organism Escherichia coli strain BTH101

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag JWV29_RS22255 Protein ID WP_000155570.1
Coordinates 4595921..4596994 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag JWV29_RS22250 Protein ID WP_000461704.1
Coordinates 4595557..4595928 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JWV29_RS22215 (4590758) 4590758..4590910 + 153 Protein_4346 arsenate reductase -
JWV29_RS22220 (4591297) 4591297..4591764 - 468 WP_000702495.1 protein YfjT -
JWV29_RS22225 (4591788) 4591788..4592231 - 444 WP_000824222.1 lipoprotein YfjS -
JWV29_RS22230 (4592231) 4592231..4592467 - 237 WP_001144029.1 protein YpjK -
JWV29_RS22235 (4592508) 4592508..4593209 - 702 WP_000189409.1 WYL domain-containing protein -
JWV29_RS22240 (4593426) 4593426..4594247 - 822 WP_000197391.1 DUF932 domain-containing protein -
JWV29_RS22245 (4594339) 4594339..4595202 - 864 WP_000787597.1 GTPase family protein -
JWV29_RS22250 (4595557) 4595557..4595928 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
JWV29_RS22255 (4595921) 4595921..4596994 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
JWV29_RS22260 (4597136) 4597136..4597399 + 264 WP_000502050.1 DUF1524 domain-containing protein -
JWV29_RS22265 (4597759) 4597759..4599375 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
JWV29_RS22270 (4599372) 4599372..4601561 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4575478..4607358 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T194162 WP_000155570.1 NZ_CP070482:c4596994-4595921 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T194162 NZ_CP093373:c3653458-3653045 [Salmonella enterica subsp. enterica serovar Infantis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTTCTGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT194162 WP_000461704.1 NZ_CP070482:c4595928-4595557 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT194162 NZ_CP093373:c3653705-3653439 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References