257592

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1117735..1119172 Replicon chromosome
Accession NZ_CP104326
Organism Escherichia coli strain THB42-F1

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag N0470_RS05395 Protein ID WP_000155570.1
Coordinates 1118099..1119172 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag N0470_RS05390 Protein ID WP_000461704.1
Coordinates 1117735..1118106 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0470_RS05355 (1112936) 1112936..1113088 + 153 Protein_1044 arsenate reductase -
N0470_RS05360 (1113475) 1113475..1113942 - 468 WP_000702495.1 protein YfjT -
N0470_RS05365 (1113966) 1113966..1114409 - 444 WP_000824222.1 lipoprotein YfjS -
N0470_RS05370 (1114409) 1114409..1114645 - 237 WP_001144029.1 protein YpjK -
N0470_RS05375 (1114686) 1114686..1115387 - 702 WP_000189409.1 WYL domain-containing protein -
N0470_RS05380 (1115604) 1115604..1116425 - 822 WP_000197391.1 DUF932 domain-containing protein -
N0470_RS05385 (1116517) 1116517..1117380 - 864 WP_000787597.1 GTPase family protein -
N0470_RS05390 (1117735) 1117735..1118106 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
N0470_RS05395 (1118099) 1118099..1119172 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
N0470_RS05400 (1119314) 1119314..1119577 + 264 WP_000502050.1 DUF1524 domain-containing protein -
N0470_RS05405 (1119937) 1119937..1121553 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
N0470_RS05410 (1121550) 1121550..1123739 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1097656..1129536 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T257592 WP_000155570.1 NZ_CP104326:c1119172-1118099 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT257592 WP_000461704.1 NZ_CP104326:c1118106-1117735 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References