Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2723806..2724473 Replicon chromosome
Accession NZ_CP076404
Organism Escherichia coli strain K-12 substr. AB1157

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag KJY95_RS13280 Protein ID WP_001094400.1
Coordinates 2724144..2724473 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag KJY95_RS13275 Protein ID WP_000072690.1
Coordinates 2723806..2724123 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJY95_RS13245 (2718858) 2718858..2719867 - 1010 Protein_2591 arsenic transporter -
KJY95_RS13250 (2720009) 2720009..2721712 + 1704 WP_001698958.1 protein YfjW -
KJY95_RS13255 (2722284) 2722284..2722507 + 224 Protein_2593 DUF905 domain-containing protein -
KJY95_RS13260 (2722610) 2722610..2723068 + 459 WP_000211841.1 antirestriction protein -
KJY95_RS13265 (2723077) 2723077..2723559 + 483 WP_001407480.1 RadC family protein -
KJY95_RS13270 (2723568) 2723568..2723768 + 201 WP_001395452.1 DUF987 domain-containing protein -
KJY95_RS13275 (2723806) 2723806..2724123 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KJY95_RS13280 (2724144) 2724144..2724473 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
KJY95_RS13285 (2724837) 2724837..2729417 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2702850..2744735 41885


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T205641 WP_001094400.1 NZ_CP076404:2724144-2724473 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T205641 NZ_CP101784:328118-328486 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT205641 WP_000072690.1 NZ_CP076404:2723806-2724123 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT205641 NZ_CP101784:327900-328121 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References