197277

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 4005999..4006666 Replicon chromosome
Accession NZ_CP072054
Organism Escherichia coli strain K1508

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag J5N95_RS19255 Protein ID WP_001094400.1
Coordinates 4005999..4006328 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag J5N95_RS19260 Protein ID WP_000072690.1
Coordinates 4006349..4006666 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5N95_RS19250 (4001055) 4001055..4005635 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
J5N95_RS19255 (4005999) 4005999..4006328 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
J5N95_RS19260 (4006349) 4006349..4006666 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
J5N95_RS19265 (4006704) 4006704..4006904 - 201 WP_001395452.1 DUF987 domain-containing protein -
J5N95_RS19270 (4006913) 4006913..4007395 - 483 WP_001407480.1 RadC family protein -
J5N95_RS19275 (4007404) 4007404..4007862 - 459 WP_000211841.1 antirestriction protein -
J5N95_RS19280 (4007965) 4007965..4008188 - 224 Protein_3805 DUF905 family protein -
J5N95_RS19285 (4008760) 4008760..4010463 - 1704 WP_000896263.1 protein YfjW -
J5N95_RS19290 (4010605) 4010605..4011614 + 1010 Protein_3807 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3996347..4028227 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T197277 WP_001094400.1 NZ_CP072054:c4006328-4005999 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T197277 NZ_CP095859:36132-36548 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT197277 WP_000072690.1 NZ_CP072054:c4006666-4006349 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT197277 NZ_CP095859:35905-36135 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References