194805

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 792336..793773 Replicon chromosome
Accession NZ_CP070962
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag JTR57_RS03890 Protein ID WP_000155570.1
Coordinates 792700..793773 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag JTR57_RS03885 Protein ID WP_000461704.1
Coordinates 792336..792707 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS23315 (787537) 787537..787689 + 153 Protein_751 arsenate reductase -
JTR57_RS03855 (788076) 788076..788543 - 468 WP_000702495.1 protein YfjT -
JTR57_RS03860 (788567) 788567..789010 - 444 WP_000824222.1 lipoprotein YfjS -
JTR57_RS03865 (789010) 789010..789246 - 237 WP_001144029.1 protein YpjK -
JTR57_RS03870 (789287) 789287..789988 - 702 WP_000189409.1 WYL domain-containing protein -
JTR57_RS03875 (790205) 790205..791026 - 822 WP_000197391.1 DUF932 domain-containing protein -
JTR57_RS03880 (791118) 791118..791981 - 864 WP_000787597.1 GTPase family protein -
JTR57_RS03885 (792336) 792336..792707 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
JTR57_RS03890 (792700) 792700..793773 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
JTR57_RS03895 (793915) 793915..794178 + 264 WP_000502050.1 DUF1524 domain-containing protein -
JTR57_RS03900 (794538) 794538..796154 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
JTR57_RS03905 (796151) 796151..798340 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 772259..804137 31878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T194805 WP_000155570.1 NZ_CP070962:c793773-792700 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T194805 NZ_CP094082:610779-611051 [Helicobacter pylori]
ATGCTGATGATTGAAACCAGTAAAAAATTTGATAAGGATCTTAAAATTCTTGTTAAAAACGGGTTTGATTTAAAGCTTTT
ATATAAAGTGGTTGAAAATTTAGCCAAAGAGCAACCCCTAGCTCCCAAATACAAAGACCACCCGCTCAAAGGCGCTTTAA
AAGATTTTAGGGAATGCCACCTGAAACCGGATTTATTGCTTGTCTATCAAATTAAAAAACAAGAAAACACTCTTTTTTTA
GTAAGGTTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT194805 WP_000461704.1 NZ_CP070962:c792707-792336 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT194805 NZ_CP094082:610481-610792 [Helicobacter pylori]
ATGCCTAACTCTCCCACCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTGACTTTTTAAATTCTATCAAGAC
CAAACAAAAAAGAGCGTTAGAAAAATTGAAAGAAATCCAAGCTCAAAAACAAAGAATCAAAAAAGCGCTCCAATTTAAAG
CGCTCCATTTAACTGAAAACGGATACACCATAGAAGAAGAGCGAGAAATCTTAGCAAGGGCTAAAGACACCAAGAACCAC
CTTTGTTTTAAAAGCGTAGAGGATTTTAGAAAGCATTGTGAAAACTTAAGACTTAAAAATGCTGATGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
AlphaFold DB A0A373F4G4

References