137865

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1405283..1406720 Replicon chromosome
Accession NZ_CP043211
Organism Escherichia coli O16:H48 strain PG20180050

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag FYA05_RS06755 Protein ID WP_000155570.1
Coordinates 1405647..1406720 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag FYA05_RS06750 Protein ID WP_000461704.1
Coordinates 1405283..1405654 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA05_RS06715 1400484..1400798 + 315 WP_000065803.1 arsenate reductase -
FYA05_RS06720 1401023..1401490 - 468 WP_000702495.1 hypothetical protein -
FYA05_RS06725 1401514..1401957 - 444 WP_000824222.1 hypothetical protein -
FYA05_RS06730 1401957..1402193 - 237 WP_001144029.1 hypothetical protein -
FYA05_RS06735 1402234..1402935 - 702 WP_000189409.1 WYL domain-containing protein -
FYA05_RS06740 1403152..1403973 - 822 WP_000197391.1 DUF945 domain-containing protein -
FYA05_RS06745 1404065..1404928 - 864 WP_000787597.1 GTPase family protein -
FYA05_RS06750 1405283..1405654 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
FYA05_RS06755 1405647..1406720 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
FYA05_RS06760 1406862..1407125 + 264 WP_000502050.1 DUF1524 domain-containing protein -
FYA05_RS06765 1407485..1409101 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
FYA05_RS06770 1409098..1411287 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1385204..1417084 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T137865 WP_000155570.1 NZ_CP043211:c1406720-1405647 [Escherichia coli O16:H48]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T137865 NZ_CP057093:c4449975-4449868 [Escherichia fergusonii]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT137865 WP_000461704.1 NZ_CP043211:c1405654-1405283 [Escherichia coli O16:H48]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT137865 NZ_CP057093:4450033-4450087 [Escherichia fergusonii]
CAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References