218275

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1118413..1119850 Replicon chromosome
Accession NZ_CP084209
Organism Escherichia coli strain EC2648-R2

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag LDB16_RS05400 Protein ID WP_000155570.1
Coordinates 1118777..1119850 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag LDB16_RS05395 Protein ID WP_000461704.1
Coordinates 1118413..1118784 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB16_RS05360 (1113614) 1113614..1113766 + 153 Protein_1053 arsenate reductase -
LDB16_RS05365 (1114153) 1114153..1114620 - 468 WP_000702495.1 protein YfjT -
LDB16_RS05370 (1114644) 1114644..1115087 - 444 WP_000824222.1 lipoprotein YfjS -
LDB16_RS05375 (1115087) 1115087..1115323 - 237 WP_001144029.1 protein YpjK -
LDB16_RS05380 (1115364) 1115364..1116065 - 702 WP_000189409.1 WYL domain-containing protein -
LDB16_RS05385 (1116282) 1116282..1117103 - 822 WP_000197391.1 DUF932 domain-containing protein -
LDB16_RS05390 (1117195) 1117195..1118058 - 864 WP_000787597.1 GTPase family protein -
LDB16_RS05395 (1118413) 1118413..1118784 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
LDB16_RS05400 (1118777) 1118777..1119850 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
LDB16_RS05405 (1119992) 1119992..1120255 + 264 WP_000502050.1 DUF1524 domain-containing protein -
LDB16_RS05410 (1120615) 1120615..1122231 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
LDB16_RS05415 (1122228) 1122228..1124417 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1098334..1130214 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T218275 WP_000155570.1 NZ_CP084209:c1119850-1118777 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T218275 NZ_CP115715:135743-136159 [Klebsiella pneumoniae]
GTGAACAAAATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATTACCTACGCCGAGATGCGCTTCGGCGCCACCGGGC
CGAAGGCCGCGCCGCGTCACGTCCAGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCATGGCCGGCACGCCGATTGGCCCGAACGACACGGCGATTGC
CGGACACGCCATCGCCGCCGGCGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTGCCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT218275 WP_000461704.1 NZ_CP084209:c1118784-1118413 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT218275 NZ_CP115715:135516-135746 [Klebsiella pneumoniae]
ATGCGCACAGTCTCTATTTTTAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References