218244

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1927554..1928991 Replicon chromosome
Accession NZ_CP084207
Organism Escherichia coli strain EC2648-R1-1

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag LDB15_RS09220 Protein ID WP_000155570.1
Coordinates 1927918..1928991 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag LDB15_RS09215 Protein ID WP_000461704.1
Coordinates 1927554..1927925 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB15_RS09180 (1922755) 1922755..1922907 + 153 Protein_1786 arsenate reductase -
LDB15_RS09185 (1923294) 1923294..1923761 - 468 WP_000702495.1 protein YfjT -
LDB15_RS09190 (1923785) 1923785..1924228 - 444 WP_000824222.1 lipoprotein YfjS -
LDB15_RS09195 (1924228) 1924228..1924464 - 237 WP_001144029.1 protein YpjK -
LDB15_RS09200 (1924505) 1924505..1925206 - 702 WP_000189409.1 WYL domain-containing protein -
LDB15_RS09205 (1925423) 1925423..1926244 - 822 WP_000197391.1 DUF932 domain-containing protein -
LDB15_RS09210 (1926336) 1926336..1927199 - 864 WP_000787597.1 GTPase family protein -
LDB15_RS09215 (1927554) 1927554..1927925 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
LDB15_RS09220 (1927918) 1927918..1928991 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
LDB15_RS09225 (1929133) 1929133..1929396 + 264 WP_000502050.1 DUF1524 domain-containing protein -
LDB15_RS09230 (1929756) 1929756..1931372 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
LDB15_RS09235 (1931369) 1931369..1933558 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1907475..1939355 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T218244 WP_000155570.1 NZ_CP084207:c1928991-1927918 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T218244 NZ_CP115712:76105-76509 [Kosakonia pseudosacchari]
ATGCTCAAATTTATGCTGGATACGAATATTTGCATTTTCACTATCAAGAACAAACCTGAAATCGTCAGGCAGGCTTTTAA
TCAGCACAGCGGGCAGATGTGTATCAGCTCTGTCACCTTGATGGAGCTGATTTATGGTGCCGAGAAATCGGCCATCCCCG
AAAAAAATTTGCGGGTTGTGGAAGGGTTTACAGCACGCCTGGAAGTGCTGCCATATGGTGTCGATGCTGCTGTTCATACA
GGCCAGTTGCGTGCGGAGTTAGCCAGAGCAGGTACGCCAGTAGGGCCATATGATGCCATGATTGGCGCACATGCTCGCTC
ACTGGGACTGGTACTTGTCAGCAATAACACTCGCGAGTTTGCTCGTATACCTGGCTTGCGCTTGCAGGACTGGACCTCGC
ATTAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT218244 WP_000461704.1 NZ_CP084207:c1927925-1927554 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT218244 NZ_CP115712:75863-76105 [Kosakonia pseudosacchari]
ATGGAGGCGCGTGTGGAAAAAACGACACTTTTCAAAAGCAACCGGAGTCAGGCCGTGCGTTTGCCAAAGGCAGTTGCGCT
GCCGGAGGACGTGAAACATGTTGATATCATTGCCGTAGGAAGAACACGCATCATTACCCCCTCCGGGGAGGCCTGGGATA
GCTGGTTTGATGGTGAAAGTGCGTCACCAGATTTTATGGCTGCACGGGAGCAGCCAGAGGAGCAAGACAGGGAAGACTTT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References