209815

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1118551..1119218 Replicon chromosome
Accession NZ_CP079735
Organism Escherichia coli strain GB2005

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag KYX12_RS05390 Protein ID WP_001094400.1
Coordinates 1118551..1118880 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag KYX12_RS05395 Protein ID WP_000072690.1
Coordinates 1118901..1119218 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX12_RS05385 (1113607) 1113607..1118187 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
KYX12_RS05390 (1118551) 1118551..1118880 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
KYX12_RS05395 (1118901) 1118901..1119218 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KYX12_RS05400 (1119256) 1119256..1119456 - 201 WP_001395452.1 DUF987 domain-containing protein -
KYX12_RS05405 (1119465) 1119465..1119947 - 483 WP_001407480.1 RadC family protein -
KYX12_RS05410 (1119956) 1119956..1120414 - 459 WP_000211841.1 antirestriction protein -
KYX12_RS05415 (1120517) 1120517..1120701 - 185 Protein_1058 DUF905 domain-containing protein -
KYX12_RS05420 (1121312) 1121312..1123015 - 1704 WP_000896263.1 protein YfjW -
KYX12_RS05425 (1123157) 1123157..1124166 + 1010 Protein_1060 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1108899..1140779 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T209815 WP_001094400.1 NZ_CP079735:c1118880-1118551 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T209815 NZ_CP104316:98483-98926 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT209815 WP_000072690.1 NZ_CP079735:c1119218-1118901 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT209815 NZ_CP104316:98256-98486 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References