Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1917127..1917794 Replicon chromosome
Accession NZ_CP084207
Organism Escherichia coli strain EC2648-R1-1

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag LDB15_RS09140 Protein ID WP_001094400.1
Coordinates 1917127..1917456 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag LDB15_RS09145 Protein ID WP_000072690.1
Coordinates 1917477..1917794 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDB15_RS09135 (1912183) 1912183..1916763 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
LDB15_RS09140 (1917127) 1917127..1917456 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
LDB15_RS09145 (1917477) 1917477..1917794 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LDB15_RS09150 (1917832) 1917832..1918032 - 201 WP_001395452.1 DUF987 domain-containing protein -
LDB15_RS09155 (1918041) 1918041..1918523 - 483 WP_001407480.1 RadC family protein -
LDB15_RS09160 (1918532) 1918532..1918990 - 459 WP_000211841.1 antirestriction protein -
LDB15_RS09165 (1919093) 1919093..1919277 - 185 Protein_1783 DUF905 family protein -
LDB15_RS09170 (1919888) 1919888..1921591 - 1704 WP_000896263.1 protein YfjW -
LDB15_RS09175 (1921733) 1921733..1922742 + 1010 Protein_1785 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1907475..1939355 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T218243 WP_001094400.1 NZ_CP084207:c1917456-1917127 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T218243 NZ_CP115709:c35039-34623 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT218243 WP_000072690.1 NZ_CP084207:c1917794-1917477 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT218243 NZ_CP115709:c35266-35036 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References