186390

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1121602..1123039 Replicon chromosome
Accession NZ_CP067994
Organism Escherichia coli strain CV601

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag JJT18_RS05380 Protein ID WP_000155570.1
Coordinates 1121966..1123039 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag JJT18_RS05375 Protein ID WP_000461704.1
Coordinates 1121602..1121973 (-) Length 124 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJT18_RS05340 1116803..1117117 + 315 WP_000065803.1 arsenate reductase -
JJT18_RS05345 1117342..1117809 - 468 WP_000702495.1 hypothetical protein -
JJT18_RS05350 1117833..1118276 - 444 WP_000824222.1 hypothetical protein -
JJT18_RS05355 1118276..1118512 - 237 WP_001144029.1 hypothetical protein -
JJT18_RS05360 1118553..1119254 - 702 WP_000189409.1 WYL domain-containing protein -
JJT18_RS05365 1119471..1120292 - 822 WP_000197391.1 DUF945 domain-containing protein -
JJT18_RS05370 1120384..1121247 - 864 WP_000787597.1 GTPase family protein -
JJT18_RS05375 1121602..1121973 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
JJT18_RS05380 1121966..1123039 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
JJT18_RS05385 1123181..1123444 + 264 WP_000502050.1 DUF1524 domain-containing protein -
JJT18_RS05390 1123804..1125420 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
JJT18_RS05395 1125417..1127606 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1101523..1133403 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T186390 WP_000155570.1 NZ_CP067994:c1123039-1121966 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T186390 NZ_CP089407:5606848-5607258 [Klebsiella michiganensis]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAGTGGTTTTCGCTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCAGGGAGAAATCAAGCTGCTGATCGACTCCCGTCTGCGCTGGCAAAAGGCG
GAGTGGGATATCATCGGCACCCCGTGGGTGATTAACAGCGGAATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGAAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGAGACCTGCGTCGGCTGGTGTTGCTAAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT186390 WP_000461704.1 NZ_CP067994:c1121973-1121602 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT186390 NZ_CP089407:5606601-5606867 [Klebsiella michiganensis]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTCAGCGATGGCGACAAAGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S


Antitoxin

Source ID Structure
AlphaFold DB A0A373F4G4

References