137439

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1492524..1493961 Replicon chromosome
Accession NZ_CP043185
Organism Escherichia coli O16:H48 strain PG20180171

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag FYA18_RS07280 Protein ID WP_000155570.1
Coordinates 1492524..1493597 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag FYA18_RS07285 Protein ID WP_000461704.1
Coordinates 1493590..1493961 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA18_RS07265 1487957..1490146 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
FYA18_RS07270 1490143..1491759 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
FYA18_RS07275 1492119..1492382 - 264 WP_000502050.1 DUF1524 domain-containing protein -
FYA18_RS07280 1492524..1493597 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
FYA18_RS07285 1493590..1493961 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
FYA18_RS07290 1494316..1495179 + 864 WP_000787597.1 GTPase family protein -
FYA18_RS07295 1495271..1496092 + 822 WP_000197391.1 DUF945 domain-containing protein -
FYA18_RS07300 1496309..1497010 + 702 WP_000189409.1 WYL domain-containing protein -
FYA18_RS07305 1497051..1497287 + 237 WP_001144029.1 hypothetical protein -
FYA18_RS07310 1497287..1497730 + 444 WP_000824222.1 hypothetical protein -
FYA18_RS07315 1497754..1498221 + 468 WP_000702495.1 hypothetical protein -
FYA18_RS07320 1498446..1498760 - 315 WP_000065803.1 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1482765..1524650 41885


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T137439 WP_000155570.1 NZ_CP043185:1492524-1493597 [Escherichia coli O16:H48]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T137439 NZ_CP056742:c66662-66411 [Enterobacter hormaechei]
ATGCGTGTTATTAGTTACAGTGAAGCCCGACAAAACCTGTCGGCAACCATGGTTCAGACGACGGAAGACCGCGCGCCGGT
GCTTATTACGCGCCAGAACGGTGAATCTTGTGTTCTGATGTCGCTGGAAGAGTACAACTCGCTTGAAGAAACCGCATACC
TGATGCGCTCCCCGGCAAACGCAAAACGTCTGATGAACTCTATCGAGAGCTTGAAATCTGGTAGCGGGAAGGAAAGGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT137439 WP_000461704.1 NZ_CP043185:1493590-1493961 [Escherichia coli O16:H48]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT137439 NZ_CP056742:c66414-66160 [Enterobacter hormaechei]
GTGAAGATCACATGGTCTGAAGAGGCATGGGAAGACTACCTCTATTGGCAGGAGACCGATAAGCGCATGGTGAGGAAAAT
CAATGAGCTTATCAAAATCTCCTGTCGCACTCCGTTTGAGGGGATCGGCAAGCCAGAGCCATTAAAACATAACCTGGCTG
GCTTCTGGTCTCGCCGAATCACCTCTGAGCATCGCATGGTCTATGCCATCACAGAAGAAGCGATCCTCATAGCTGCGTGC
CGATATCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References