260306

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1121838..1123275 Replicon chromosome
Accession NZ_CP107002
Organism Escherichia coli strain SY22C3

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag OFB02_RS05475 Protein ID WP_000155570.1
Coordinates 1122202..1123275 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag OFB02_RS05470 Protein ID WP_000461704.1
Coordinates 1121838..1122209 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OFB02_RS05435 (1117039) 1117039..1117191 + 153 Protein_1061 arsenate reductase -
OFB02_RS05440 (1117578) 1117578..1118045 - 468 WP_000702495.1 protein YfjT -
OFB02_RS05445 (1118069) 1118069..1118512 - 444 WP_000824222.1 lipoprotein YfjS -
OFB02_RS05450 (1118512) 1118512..1118748 - 237 WP_001144029.1 protein YpjK -
OFB02_RS05455 (1118789) 1118789..1119490 - 702 WP_000189409.1 WYL domain-containing protein -
OFB02_RS05460 (1119707) 1119707..1120528 - 822 WP_000197391.1 DUF932 domain-containing protein -
OFB02_RS05465 (1120620) 1120620..1121483 - 864 WP_000787597.1 GTPase family protein -
OFB02_RS05470 (1121838) 1121838..1122209 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
OFB02_RS05475 (1122202) 1122202..1123275 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
OFB02_RS05480 (1123417) 1123417..1123680 + 264 WP_000502050.1 DUF1524 domain-containing protein -
OFB02_RS05485 (1124040) 1124040..1125656 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
OFB02_RS05490 (1125653) 1125653..1127842 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1101759..1133639 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T260306 WP_000155570.1 NZ_CP107002:c1123275-1122202 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT260306 WP_000461704.1 NZ_CP107002:c1122209-1121838 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References