246493

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1742708..1744145 Replicon chromosome
Accession NZ_CP097884
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag NAG72_RS08335 Protein ID WP_000155570.1
Coordinates 1743072..1744145 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag NAG72_RS08330 Protein ID WP_000461704.1
Coordinates 1742708..1743079 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG72_RS08295 (1737909) 1737909..1738061 + 153 Protein_1600 arsenate reductase -
NAG72_RS08300 (1738448) 1738448..1738915 - 468 WP_000702495.1 protein YfjT -
NAG72_RS08305 (1738939) 1738939..1739382 - 444 WP_000824222.1 lipoprotein YfjS -
NAG72_RS08310 (1739382) 1739382..1739618 - 237 WP_001144029.1 protein YpjK -
NAG72_RS08315 (1739659) 1739659..1740360 - 702 WP_000189409.1 WYL domain-containing protein -
NAG72_RS08320 (1740577) 1740577..1741398 - 822 WP_000197391.1 DUF932 domain-containing protein -
NAG72_RS08325 (1741490) 1741490..1742353 - 864 WP_000787597.1 GTPase family protein -
NAG72_RS08330 (1742708) 1742708..1743079 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
NAG72_RS08335 (1743072) 1743072..1744145 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
NAG72_RS08340 (1744287) 1744287..1744550 + 264 WP_000502050.1 DUF1524 domain-containing protein -
NAG72_RS08345 (1744910) 1744910..1746526 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
NAG72_RS08350 (1746523) 1746523..1748712 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1722629..1754509 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T246493 WP_000155570.1 NZ_CP097884:c1744145-1743072 [Escherichia coli str. K-12 substr. MG1655]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT246493 WP_000461704.1 NZ_CP097884:c1743079-1742708 [Escherichia coli str. K-12 substr. MG1655]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References