Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1107148..1107815 Replicon chromosome
Accession NZ_CP097883
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag NAG71_RS05315 Protein ID WP_001094400.1
Coordinates 1107148..1107477 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag NAG71_RS05320 Protein ID WP_000072690.1
Coordinates 1107498..1107815 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG71_RS05310 (1102204) 1102204..1106784 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
NAG71_RS05315 (1107148) 1107148..1107477 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
NAG71_RS05320 (1107498) 1107498..1107815 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
NAG71_RS05325 (1107853) 1107853..1108053 - 201 WP_001395452.1 DUF987 domain-containing protein -
NAG71_RS05330 (1108062) 1108062..1108544 - 483 WP_001407480.1 RadC family protein -
NAG71_RS05335 (1108553) 1108553..1109011 - 459 WP_000211841.1 antirestriction protein -
NAG71_RS05340 (1109114) 1109114..1109298 - 185 Protein_1041 DUF905 domain-containing protein -
NAG71_RS05345 (1109909) 1109909..1111612 - 1704 WP_000896263.1 protein YfjW -
NAG71_RS05350 (1111754) 1111754..1112763 + 1010 Protein_1043 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1097496..1129376 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T246465 WP_001094400.1 NZ_CP097883:c1107477-1107148 [Escherichia coli str. K-12 substr. MG1655]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT246465 WP_000072690.1 NZ_CP097883:c1107815-1107498 [Escherichia coli str. K-12 substr. MG1655]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References