Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2222333..2223770 Replicon chromosome
Accession NZ_CP114896
Organism Escherichia coli strain CM93

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag O6117_RS10920 Protein ID WP_000155570.1
Coordinates 2222333..2223406 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag O6117_RS10925 Protein ID WP_000461704.1
Coordinates 2223399..2223770 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6117_RS10905 (2217766) 2217766..2219955 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
O6117_RS10910 (2219952) 2219952..2221568 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
O6117_RS10915 (2221928) 2221928..2222191 - 264 WP_000502050.1 DUF1524 domain-containing protein -
O6117_RS10920 (2222333) 2222333..2223406 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
O6117_RS10925 (2223399) 2223399..2223770 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
O6117_RS10930 (2224125) 2224125..2224988 + 864 WP_000787597.1 GTPase family protein -
O6117_RS10935 (2225080) 2225080..2225901 + 822 WP_000197391.1 DUF932 domain-containing protein -
O6117_RS10940 (2226118) 2226118..2226819 + 702 WP_000189409.1 WYL domain-containing protein -
O6117_RS10945 (2226860) 2226860..2227096 + 237 WP_001144029.1 protein YpjK -
O6117_RS10950 (2227096) 2227096..2227539 + 444 WP_000824222.1 lipoprotein YfjS -
O6117_RS10955 (2227563) 2227563..2228030 + 468 WP_000702495.1 protein YfjT -
O6117_RS10960 (2228417) 2228417..2228569 - 153 Protein_2139 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2212574..2254459 41885


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T267028 WP_000155570.1 NZ_CP114896:2222333-2223406 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT267028 WP_000461704.1 NZ_CP114896:2223399-2223770 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References