Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 781911..782578 Replicon chromosome
Accession NZ_CP070962
Organism Escherichia coli strain K-12 J53

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag JTR57_RS03810 Protein ID WP_001094400.1
Coordinates 781911..782240 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag JTR57_RS03815 Protein ID WP_000072690.1
Coordinates 782261..782578 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTR57_RS03805 (776967) 776967..781547 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
JTR57_RS03810 (781911) 781911..782240 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
JTR57_RS03815 (782261) 782261..782578 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JTR57_RS03820 (782616) 782616..782816 - 201 WP_001395452.1 DUF987 domain-containing protein -
JTR57_RS03825 (782825) 782825..783307 - 483 WP_001407480.1 RadC family protein -
JTR57_RS03830 (783316) 783316..783774 - 459 WP_000211841.1 antirestriction protein -
JTR57_RS03835 (783877) 783877..784100 - 224 Protein_748 DUF905 family protein -
JTR57_RS03840 (784670) 784670..786373 - 1704 WP_000896263.1 protein YfjW -
JTR57_RS03845 (786515) 786515..787524 + 1010 Protein_750 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 772259..804137 31878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T194804 WP_001094400.1 NZ_CP070962:c782240-781911 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T194804 NZ_CP094082:354650-354931 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTACAGAAAGCGA
TGTTGTAAGCATTGTTGATCGCCTCAAAGAGCAAAAACCACTCCAACAAAAATATTGCGATCATGCCTTAAGTGGCAATC
TTAAAGGGTTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT194804 WP_000072690.1 NZ_CP070962:c782578-782261 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT194804 NZ_CP094082:354343-354657 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCGCAATTTCTTAAACGCCATTGAAAAACAATG
GCTTAAGGCAGAAAGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAGTGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References