Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2238350..2239787 Replicon chromosome
Accession NZ_CP093221
Organism Escherichia coli strain ER2683

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag MNV48_RS10825 Protein ID WP_000155570.1
Coordinates 2238714..2239787 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag MNV48_RS10820 Protein ID WP_000461704.1
Coordinates 2238350..2238721 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNV48_RS10785 (2233551) 2233551..2233703 + 153 Protein_2100 arsenate reductase -
MNV48_RS10790 (2234090) 2234090..2234557 - 468 WP_000702495.1 protein YfjT -
MNV48_RS10795 (2234581) 2234581..2235024 - 444 WP_000824222.1 lipoprotein YfjS -
MNV48_RS10800 (2235024) 2235024..2235260 - 237 WP_001144029.1 protein YpjK -
MNV48_RS10805 (2235301) 2235301..2236002 - 702 WP_000189409.1 WYL domain-containing protein -
MNV48_RS10810 (2236219) 2236219..2237040 - 822 WP_000197391.1 DUF932 domain-containing protein -
MNV48_RS10815 (2237132) 2237132..2237995 - 864 WP_000787597.1 GTPase family protein -
MNV48_RS10820 (2238350) 2238350..2238721 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
MNV48_RS10825 (2238714) 2238714..2239787 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
MNV48_RS10830 (2239929) 2239929..2240192 + 264 WP_000502050.1 DUF1524 domain-containing protein -
MNV48_RS10835 (2240552) 2240552..2242168 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
MNV48_RS10840 (2242165) 2242165..2244354 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2218271..2250151 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T237830 WP_000155570.1 NZ_CP093221:c2239787-2238714 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T237830 NZ_LR882973:c4256173-4256000 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT237830 WP_000461704.1 NZ_CP093221:c2238721-2238350 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT237830 NZ_LR882973:c4256497-4256201 [Escherichia coli]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References