137559

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1109881..1110548 Replicon chromosome
Accession NZ_CP043193
Organism Escherichia coli O16:H48 strain PG20180063

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag FYA14_RS05345 Protein ID WP_001094400.1
Coordinates 1109881..1110210 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag FYA14_RS05350 Protein ID WP_000072690.1
Coordinates 1110231..1110548 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA14_RS05340 1104937..1109517 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
FYA14_RS05345 1109881..1110210 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
FYA14_RS05350 1110231..1110548 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FYA14_RS05355 1110586..1110786 - 201 WP_001395452.1 DUF987 domain-containing protein -
FYA14_RS05360 1110795..1111277 - 483 WP_001407480.1 RadC family protein -
FYA14_RS05365 1111286..1111744 - 459 WP_000211841.1 antirestriction protein -
FYA14_RS05370 1111847..1112070 - 224 Protein_1044 DUF905 domain-containing protein -
FYA14_RS05375 1112642..1114345 - 1704 WP_000896263.1 hypothetical protein -
FYA14_RS05380 1114487..1115496 + 1010 Protein_1046 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1100229..1132109 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T137559 WP_001094400.1 NZ_CP043193:c1110210-1109881 [Escherichia coli O16:H48]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T137559 NZ_CP043193:c1110210-1109881 [Escherichia coli O16:H48]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT137559 WP_000072690.1 NZ_CP043193:c1110548-1110231 [Escherichia coli O16:H48]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT137559 NZ_CP056809:c2079323-2079180 [Citrobacter freundii]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References