276722

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 148257..149694 Replicon chromosome
Accession NZ_CP122318
Organism Escherichia coli strain HT873X1

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag QCG45_RS00755 Protein ID WP_000155570.1
Coordinates 148621..149694 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag QCG45_RS00750 Protein ID WP_000461704.1
Coordinates 148257..148628 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCG45_RS00715 (143458) 143458..143610 + 153 Protein_136 arsenate reductase -
QCG45_RS00720 (143997) 143997..144464 - 468 WP_000702495.1 protein YfjT -
QCG45_RS00725 (144488) 144488..144931 - 444 WP_000824222.1 lipoprotein YfjS -
QCG45_RS00730 (144931) 144931..145167 - 237 WP_001144029.1 protein YpjK -
QCG45_RS00735 (145208) 145208..145909 - 702 WP_000189409.1 WYL domain-containing protein -
QCG45_RS00740 (146126) 146126..146947 - 822 WP_000197391.1 DUF932 domain-containing protein -
QCG45_RS00745 (147039) 147039..147902 - 864 WP_000787597.1 GTPase family protein -
QCG45_RS00750 (148257) 148257..148628 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
QCG45_RS00755 (148621) 148621..149694 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
QCG45_RS00760 (149836) 149836..150099 + 264 WP_000502050.1 DUF1524 domain-containing protein -
QCG45_RS00765 (150459) 150459..152075 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
QCG45_RS00770 (152072) 152072..154261 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 128178..160058 31880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T276722 WP_000155570.1 NZ_CP122318:c149694-148621 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT276722 WP_000461704.1 NZ_CP122318:c148628-148257 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References