142445

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2166303..2167740 Replicon chromosome
Accession NZ_CP045741
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag GHR40_RS10345 Protein ID WP_000155570.1
Coordinates 2166667..2167740 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag GHR40_RS10340 Protein ID WP_000461704.1
Coordinates 2166303..2166674 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHR40_RS10305 2161504..2161818 + 315 WP_000065803.1 arsenate reductase -
GHR40_RS10310 2162043..2162510 - 468 WP_000702495.1 hypothetical protein -
GHR40_RS10315 2162534..2162977 - 444 WP_000824222.1 hypothetical protein -
GHR40_RS10320 2162977..2163213 - 237 WP_001144029.1 hypothetical protein -
GHR40_RS10325 2163254..2163955 - 702 WP_000189409.1 WYL domain-containing protein -
GHR40_RS10330 2164172..2164993 - 822 WP_000197391.1 DUF945 domain-containing protein -
GHR40_RS10335 2165085..2165948 - 864 WP_000787597.1 GTPase family protein -
GHR40_RS10340 2166303..2166674 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
GHR40_RS10345 2166667..2167740 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
GHR40_RS10350 2167882..2168145 + 264 WP_000502050.1 DUF1524 domain-containing protein -
GHR40_RS10355 2168505..2170121 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
GHR40_RS10360 2170118..2172307 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2145946..2178101 32155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T142445 WP_000155570.1 NZ_CP045741:c2167740-2166667 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T142445 NZ_CP060700:c1303380-1303027 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATTCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT142445 WP_000461704.1 NZ_CP045741:c2166674-2166303 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT142445 NZ_CP060700:c1303551-1303381 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References