Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1108963..1109630 Replicon chromosome
Accession NZ_CP049112
Organism Escherichia coli strain WF5-5-T2

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag G6R06_RS05370 Protein ID WP_001094400.1
Coordinates 1108963..1109292 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag G6R06_RS05375 Protein ID WP_000072690.1
Coordinates 1109313..1109630 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6R06_RS05365 (1104019) 1104019..1108599 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
G6R06_RS05370 (1108963) 1108963..1109292 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
G6R06_RS05375 (1109313) 1109313..1109630 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
G6R06_RS05380 (1109668) 1109668..1109868 - 201 WP_001395452.1 DUF987 domain-containing protein -
G6R06_RS05385 (1109877) 1109877..1110359 - 483 WP_001407480.1 RadC family protein -
G6R06_RS05390 (1110368) 1110368..1110826 - 459 WP_000211841.1 antirestriction protein -
G6R06_RS05395 (1110839) 1110839..1110961 + 123 WP_001407482.1 hypothetical protein -
G6R06_RS05400 (1110929) 1110929..1111113 - 185 Protein_1053 DUF905 family protein -
G6R06_RS05405 (1111724) 1111724..1113427 - 1704 WP_000896263.1 protein YfjW -
G6R06_RS05410 (1113569) 1113569..1114578 + 1010 Protein_1055 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1099311..1131192 31881


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T150556 WP_001094400.1 NZ_CP049112:c1109292-1108963 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T150556 NZ_CP064794:5302-6567 [Xanthomonas oryzae pv. oryzicola]
ATGAAAAACAAGACAGACATTGCTGGCATAGCCGGACTTTCTACGTCTCATGTTTCCTTTGATGACGCAGAATCCAAAAG
CGTCCTAGATGGATCAACACAGTCTACGCCGGAGTACAAATTCCAGACCCCGCTTGATCAGTTGCCGGCTAGGCCCGCTG
GTCCGAAGAGCAGTAACACAACTGATGCTTACATTCATGAATCACTGCCGCATTCGGTTCCAATCGCAGGTGCATCGACC
TCCGCGGTGACACATCAACAGGCTACTCAGCGTAAAGGCACCGATGAGATCTATGGTTTGGGCAGCCTCCCATCTGCTGG
TCCAGGGCGATGGGAGTATCTTGCGAATCCGGGGAACTGGCATCCCGAGCGCCGTAAGTTACACGAAAAGCTATTAGATC
AAGCGCGATCTTCTGCCTTGACCCTGGCAGAATCCCTTGAGAGTGATGGCTGCCAACCGACATTGTTTGCGCTGCGCGGA
AATACCGCAACGGGTAAAACACGGATTGCGACAAAAAAGATCCCGGTGCTCGCAGCAGCTCTGAAGAAGACAGCTGGGAA
AGGTTGCGTGAATCCAGATGTATTCAAGAGTTCGCTTGCGAAATCCGAGACAGGCGCCAAGATATTTAGTTCGGCACAAG
TGCATAGCGAGTCATCTTTTCTTGCCGACCGGTTTGAAGGCGGACTTCGCTCTCAAAAGACGGGCAGCGGTGCAATCGCT
AGCATTGTTGTCGACAAGCGCTTGTCAAGAGAGTACGAAATTGATAGCTACATACAACTAGCGAAAGAAACCGGTAGAAA
AGTGGAATTGTGCGATATCGACGCGCCCCTAGAGAATTCTTTGGTGGGAGTTTTGCAGCGAAAACCGGAAGGTGAAGATC
CTAGGCCTCCGTACCCTGTAGTCTCGAGTGGATTTGTAGCCGTACGGAGCAATAGAATGTACGTGATAGACAGGTTCATT
GCCGATCCGAGCCTGGGAAACTACCGACTATTCGGAACAGCTGAAGATGGTGAGAAGGTCATGGTTGCTAGCGTGATTGG
CGGTGAGTTTAGTGTAGAGAATGCTGAGTTGTATGAAAAAATCACGTCCCCACAGCTCTCGGTTACGGGCGATTTGGCTG
ATAAGGTAATCGACAAGGAATTGATTGATCGTCTGGAAAATAATATAGCTGACCCCGAGCGGGCGGCCAAGACGCGAGCC
GCCCTGGAAAAATACAGCGGTAAGTCATGGTCGGCCGCGCTAGCTGCTCACAGCGAGCTAATTTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT150556 WP_000072690.1 NZ_CP049112:c1109630-1109313 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT150556 NZ_CP064794:6585-6881 [Xanthomonas oryzae pv. oryzicola]
ATGAAAACTTTGACAGGCGCAGACGCACTCGAATTTCATAAGAAGCTTAAGGAACGCAATAAAGCCTTACATGCTTCCGA
CCTCGAGTTAGCACTGGTGCACGCCGATGCTGTCGGCAAAGAACGGTTCGATCTCGAGGAGCTGGAGAAAATTTGCGATA
CCAGCGATGCGGGGCGCCTTACTGATGCGAAAGAGCGAAACGACATATATGAACGGATGTACTACGTTGAGTATCCCAAC
GTTATGACGTTAAAGGAATTCGCTCATATCGTCGAGACACTTTTCTCGTGGTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References