209843

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1128985..1130422 Replicon chromosome
Accession NZ_CP079736
Organism Escherichia coli strain GB2005-delet-a-f

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag KYX13_RS05470 Protein ID WP_000155570.1
Coordinates 1129349..1130422 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag KYX13_RS05465 Protein ID WP_000461704.1
Coordinates 1128985..1129356 (-) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX13_RS22215 (1124186) 1124186..1124338 + 153 Protein_1061 arsenate reductase -
KYX13_RS05435 (1124725) 1124725..1125192 - 468 WP_000702495.1 protein YfjT -
KYX13_RS05440 (1125216) 1125216..1125659 - 444 WP_000824222.1 lipoprotein YfjS -
KYX13_RS05445 (1125659) 1125659..1125895 - 237 WP_001144029.1 protein YpjK -
KYX13_RS05450 (1125936) 1125936..1126637 - 702 WP_000189409.1 WYL domain-containing protein -
KYX13_RS05455 (1126854) 1126854..1127675 - 822 WP_000197391.1 DUF932 domain-containing protein -
KYX13_RS05460 (1127767) 1127767..1128630 - 864 WP_000787597.1 GTPase family protein -
KYX13_RS05465 (1128985) 1128985..1129356 - 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
KYX13_RS05470 (1129349) 1129349..1130422 - 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
KYX13_RS05475 (1130564) 1130564..1130827 + 264 WP_000502050.1 DUF1524 domain-containing protein -
KYX13_RS05480 (1131187) 1131187..1132803 + 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
KYX13_RS05485 (1132800) 1132800..1134989 + 2190 WP_000136402.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1108906..1139845 30939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T209843 WP_000155570.1 NZ_CP079736:c1130422-1129349 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T209843 NZ_CP104326:3638421-3638699 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT209843 WP_000461704.1 NZ_CP079736:c1129356-1128985 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT209843 NZ_CP104326:3638158-3638418 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
AlphaFold DB A0A373F4G4

References